Read the following and answer questions from \( 15(\mathrm{i}) \) to \( 15(\mathrm{v}) \). Rest...

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Read the following and answer questions from \( 15(\mathrm{i}) \) to \( 15(\mathrm{v}) \).

Restriction endonuclease was isolated for the first time by W.Arberin 1962 inbacteria. Restrictionendonucleases cut the DNA duplex at specific points therefore they are also calledmolecularscissors orbiologicalscissors. Threetypes of restriction endonucleases are Type I, Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoRI recognises the base sequence GAATTC in DNA duplex and cut strands between \( \mathrm{G} \) and \( \mathrm{A} \).
(iv) Restrictionendonucleasesarealsocalledasmolecular or biological scissors because
(A) they cleave base pairs of DNA only at their terminal ends
(B) they cleave one or both the strands of DNA
(C) they act only on single stranded DNA
(D) none of these.
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