| 1. |  Markiplier | WHAT IS THAT!? | Binding of Isaac - Part 1 | 66,058 | Let's Play |
| 2. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 1 [Reborn] | 39,019 | Let's Play |
| 3. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 1 [Genesis] | 22,845 | Let's Play |
| 4. | YuB | the isaac BACKWARDS challenge | 19,764 | Let's Play |
| 5. | Hutts | BROKEN 1 FIRE RATE BRIMSTONE - Alphabirth Pack 3 (MOD) | 19,122 | Let's Play |
| 6. | YuB | UNLOCKING THE FORGOTTEN! (Binding Of Isaac) | 18,760 | Let's Play |
| 7. | YuB | EPIC RUN AS THE FORGOTTEN! (Binding Of Isaac) | 18,394 | Let's Play |
| 8. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 1 [Bethany] | 16,827 | Let's Play |
| 9. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 1 [Arrival] | 16,765 | Let's Play |
| 10. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 145 [The Lost] | 16,095 | Let's Play |
| 11. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 2 [Synergy] | 15,622 | Let's Play |
| 12. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 2 [Shh] | 14,111 | Let's Play |
| 13. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 3 [Azazel] | 13,343 | Let's Play |
| 14. | YuB | APOLLYON VS ULTRA GREEDIER (binding of isaac) | 12,760 | Let's Play |
| 15. | YuB | ok but apollyon is kinda great (binding of isaac) | 12,510 | Let's Play |
| 16. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 3 [Greed] | 11,852 | Let's Play |
| 17. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 2 [Great Gideon] | 11,849 | Let's Play |
| 18. | YuB | isaac finds his long lost family! (adorable) | 11,292 | Let's Play |
| 19. | Hutts | NEW CHARACTER, THE FORGOTTEN - Hutts Plays Afterbirth+ | 11,047 | Let's Play |
| 20. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 170 [Surplus] | 10,968 | Let's Play |
| 21. | OneyPlays | Oney Plays The Binding of Isaac (Complete Series) | 10,918 | Let's Play |
| 22. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 195 [Firework] | 10,862 | Let's Play |
| 23. | YuB | most satisfying isaac run ever | 10,651 | Let's Play |
| 24. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 4 [Eden] | 10,456 | Let's Play |
| 25. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 5 [Total Control] | 10,338 | Let's Play |
| 26. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 20 [Ultra Hard] | 10,192 | Let's Play |
| 27. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 4 [Giant Strength] | 10,145 | Let's Play |
| 28. | YuB | BUMBO IS INSANE (Binding Of Isaac) | 10,095 | Let's Play |
| 29. | YuB | ISAAC - I beat delirium without touching my controller lol | 9,930 | Let's Play |
| 30. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 6 [Medulla Oblongata] | 9,827 | Let's Play |
| 31. | YuB | THE NEW ISAAC GAME IS OUT AKSLJDHFLAKSJDHFLKAS | 9,752 | Let's Play |
| 32. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 7 [What Goes Around Comes Around] | 9,674 | Let's Play |
| 33. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 3 [Purify] | 9,548 | Let's Play |
| 34. | Hutts | 10000 IQ plays lead to OP RUN! (Playable Duke) | 9,481 | Let's Play |
| 35. | Northernlion | Baby - The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 1779 | 9,401 | Let's Play |
| 36. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 4 [Re-Eden] | 8,985 | Let's Play |
| 37. | OneyPlays | Oney Plays The Binding of Issac - Ep 1 - Mayonnaise | 8,760 | Let's Play |
| 38. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 6 [Tiny Knife] | 8,729 | Let's Play |
| 39. | Hutts | Not for Kids - Hutts Plays DON'T SUE ME | 8,503 | Let's Play |
| 40. | YuB | the keeper + greed mode = AKSLJDHFLAKSJDF | 8,478 | Let's Play |
| 41. | YuB | isaac perfect accuracy challenge (this sucks) | 8,427 | Let's Play |
| 42. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 8 [Stinkbombs] | 8,383 | Let's Play |
| 43. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 34 [Ultra Hard III] | 8,376 | Let's Play |
| 44. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 652 [Wax] | 8,341 | Let's Play |
| 45. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 62 [My Best Run Ever?] | 8,334 | Let's Play |
| 46. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 2 [Apollyon] | 8,328 | Let's Play |
| 47. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 5 [Objective] | 8,182 | Let's Play |
| 48. | Hutts | NULL - The Binding of Isaac ALPHABIRTH Mod Pack | 8,084 | Let's Play |
| 49. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 39 [Revelation] | 8,046 | Let's Play |
| 50. | Sinvicta | ACTUAL BIG BRAIN PLAYS?! - The Binding Of Isaac: Afterbirth+ #1063 | 7,936 | Let's Play |
| 51. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 60 [Don't] | 7,916 | Let's Play |
| 52. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 7 [Jacob] | 7,860 | Let's Play |
| 53. | YuB | MOST OP ISAAC RUN 2019 | 7,819 | Let's Play |
| 54. | Hutts | Endor - Alphabirth Mod Pack (Afterbirth+) | 7,815 | Let's Play |
| 55. | Hutts | Can UNBELIEVABLE Luck Help me BEAT this Challenge? | 7,800 | Let's Play |
| 56. | YuB | isaac: the ultimate choke | 7,661 | Let's Play |
| 57. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 21 [Lost In Space] | 7,659 | Let's Play |
| 58. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 9 [Caviar] | 7,531 | Let's Play |
| 59. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 20 [Edenomics] | 7,475 | Let's Play |
| 60. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 11 [Restart] | 7,464 | Let's Play |
| 61. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 5 [Confidence] | 7,437 | Let's Play |
| 62. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 6 [Accomplished] | 7,416 | Let's Play |
| 63. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 23 [Floss] | 7,298 | Let's Play |
| 64. | YuB | TINY ISAAC VS THE LAMB | 7,251 | Let's Play |
| 65. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 29 [Speed!] | 7,067 | Let's Play |
| 66. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 24 [Ringo] | 7,013 | Let's Play |
| 67. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 22 [Devout] | 6,991 | Let's Play |
| 68. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 12 [April's Fool] | 6,955 | Let's Play |
| 69. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 112 [Uno] | 6,899 | Let's Play |
| 70. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 17 [Resurrection] | 6,889 | Let's Play |
| 71. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 23 [IV] | 6,857 | Let's Play |
| 72. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 21 [Introducing] | 6,821 | Let's Play |
| 73. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 126 [Holiday] | 6,791 | Let's Play |
| 74. | YuB | we did it boys (head trauma challenge) | 6,784 | Let's Play |
| 75. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 5 [Strawman] | 6,761 | Let's Play |
| 76. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 7 [Daily] | 6,755 | Let's Play |
| 77. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 3 [Greedier] | 6,737 | Let's Play |
| 78. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 587 [Triple-Double] | 6,725 | Let's Play |
| 79. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 17 [Delivered] | 6,722 | Let's Play |
| 80. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 8 [Impatience] | 6,711 | Let's Play |
| 81. | SuperMega | SuperMega Plays BINDING OF ISAAC - EP 1: Why Cockroaches Are Gross | 6,689 | Let's Play |
| 82. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 666 [Satanic] | 6,657 | Let's Play |
| 83. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 10 [Greeden] | 6,631 | Let's Play |
| 84. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 17 [Lost Boys] | 6,631 | Let's Play |
| 85. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 10 [Blastocysts] | 6,619 | Let's Play |
| 86. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 13 [Old Friends] | 6,593 | Let's Play |
| 87. | YuB | THE FORGOTTEN BOSS RUSH (isaac) | 6,565 | Let's Play |
| 88. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 12 [Trick or Treat] | 6,536 | Let's Play |
| 89. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 16 [Sea Legs] | 6,519 | Let's Play |
| 90. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 143 [Crest] | 6,490 | Let's Play |
| 91. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 9 [Incubus] | 6,446 | Let's Play |
| 92. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 10 [Hellraiser] | 6,442 | Let's Play |
| 93. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 129 [Redemption] | 6,429 | Let's Play |
| 94. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 30 [Ultra Hard II] | 6,417 | Let's Play |
| 95. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 8 [Lillith] | 6,408 | Let's Play |
| 96. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 4 [Smelted] | 6,358 | Let's Play |
| 97. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 13 [V] | 6,320 | Let's Play |
| 98. | YuB | isaac is the BOMB | 6,228 | Let's Play |
| 99. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 223 [Nolte] | 6,226 | Let's Play |
| 100. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 14 [Cursed] | 6,224 | Let's Play |
