| 1. |  Markiplier | WHAT IS THAT!? | Binding of Isaac - Part 1 | 3,478,826 | Let's Play |
| 2. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 1 [Reborn] | 2,535,496 | Let's Play |
| 3. | YuB | UNLOCKING THE FORGOTTEN! (Binding Of Isaac) | 1,737,256 | Let's Play |
| 4. | YuB | EPIC RUN AS THE FORGOTTEN! (Binding Of Isaac) | 1,473,060 | Let's Play |
| 5. | Hutts | BROKEN 1 FIRE RATE BRIMSTONE - Alphabirth Pack 3 (MOD) | 1,369,384 | Let's Play |
| 6. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 1 [Genesis] | 1,084,494 | Let's Play |
| 7. | OneyPlays | Oney Plays The Binding of Isaac (Complete Series) | 980,139 | Let's Play |
| 8. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 2 [Synergy] | 960,313 | Let's Play |
| 9. | YuB | isaac finds his long lost family! (adorable) | 876,432 | Let's Play |
| 10. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 3 [Azazel] | 783,190 | Let's Play |
| 11. | YuB | ok but apollyon is kinda great (binding of isaac) | 746,043 | Let's Play |
| 12. | YuB | APOLLYON VS ULTRA GREEDIER (binding of isaac) | 699,229 | Let's Play |
| 13. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 2 [Shh] | 695,314 | Let's Play |
| 14. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 4 [Eden] | 669,212 | Let's Play |
| 15. | YuB | TINY ISAAC VS THE LAMB | 655,658 | Let's Play |
| 16. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 1 [Arrival] | 642,149 | Let's Play |
| 17. | YuB | BUMBO IS INSANE (Binding Of Isaac) | 640,294 | Let's Play |
| 18. | Hutts | NEW CHARACTER, THE FORGOTTEN - Hutts Plays Afterbirth+ | 639,114 | Let's Play |
| 19. | Hutts | NULL - The Binding of Isaac ALPHABIRTH Mod Pack | 596,665 | Let's Play |
| 20. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 5 [Total Control] | 592,663 | Let's Play |
| 21. | YuB | the keeper + greed mode = AKSLJDHFLAKSJDF | 588,819 | Let's Play |
| 22. | YuB | most satisfying isaac run ever | 568,388 | Let's Play |
| 23. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 1 [Bethany] | 566,290 | Let's Play |
| 24. | YuB | ISAAC - I beat delirium without touching my controller lol | 564,836 | Let's Play |
| 25. | Hutts | Endor - Alphabirth Mod Pack (Afterbirth+) | 554,834 | Let's Play |
| 26. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 3 [Greed] | 553,911 | Let's Play |
| 27. | YuB | BEATING MOM'S HEART & UNLOCKING EDEN | The Binding Of Isaac Afterbirth Gameplay | 524,525 | Let's Play |
| 28. | Annoying Orange Gaming | Gaming Grape Plays - The Binding of Isaac: REBIRTH | 518,417 | Let's Play |
| 29. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 145 [The Lost] | 509,928 | Let's Play |
| 30. | Hutts | [Breaking] BASE MENT Easter Egg Seed Stream | 507,045 | Let's Play |
| 31. | YuB | the isaac BACKWARDS challenge | 499,923 | Let's Play |
| 32. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 4 [Re-Eden] | 491,150 | Let's Play |
| 33. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 6 [Medulla Oblongata] | 490,760 | Let's Play |
| 34. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 62 [My Best Run Ever?] | 462,879 | Let's Play |
| 35. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 7 [What Goes Around Comes Around] | 455,439 | Let's Play |
| 36. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 6 [Tiny Knife] | 453,357 | Let's Play |
| 37. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 8 [Stinkbombs] | 425,785 | Let's Play |
| 38. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 10 [Hellraiser] | 416,820 | Let's Play |
| 39. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 2 [Great Gideon] | 410,220 | Let's Play |
| 40. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 5 [Objective] | 400,124 | Let's Play |
| 41. | YuB | isaac: the ultimate choke | 396,121 | Let's Play |
| 42. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 9 [Caviar] | 395,602 | Let's Play |
| 43. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 2 [Apollyon] | 393,899 | Let's Play |
| 44. | YuB | MOST OP ISAAC RUN 2019 | 391,447 | Let's Play |
| 45. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 7 [Daily] | 378,536 | Let's Play |
| 46. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 10 [Greeden] | 370,735 | Let's Play |
| 47. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 8 [Lillith] | 367,951 | Let's Play |
| 48. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 39 [Revelation] | 365,520 | Let's Play |
| 49. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 12 [Trick or Treat] | 350,933 | Let's Play |
| 50. | YuB | THE FORGOTTEN BOSS RUSH (isaac) | 350,578 | Let's Play |
| 51. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 3 [Purify] | 347,577 | Let's Play |
| 52. | YuB | we did it boys (head trauma challenge) | 347,364 | Let's Play |
| 53. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 20 [Edenomics] | 346,922 | Let's Play |
| 54. | Table | Table plays - "Gornax" or, Giant Enemy Crab | 345,565 | Let's Play |
| 55. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 9 [Incubus] | 342,543 | Let's Play |
| 56. | Hutts | MOMMY HELP ME - Custom Afterbirth Challenge | 341,777 | Let's Play |
| 57. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 5 [Strawman] | 341,382 | Let's Play |
| 58. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 13 [Old Friends] | 333,855 | Let's Play |
| 59. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 16 [Sea Legs] | 333,505 | Let's Play |
| 60. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 23 [Floss] | 333,422 | Let's Play |
| 61. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 19 [Defying Gravity] | 331,853 | Let's Play |
| 62. | YuB | FIRST TIME FIGHTING MEGA SATAN (The Binding Of Isaac) | 331,358 | Let's Play |
| 63. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 87 [Purist] | 329,204 | Let's Play |
| 64. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 11 [Restart] | 328,227 | Let's Play |
| 65. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 29 [Speed!] | 326,506 | Let's Play |
| 66. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 4 [Giant Strength] | 321,358 | Let's Play |
| 67. | YuB | THE ALMIGHTY CANDLE | The Binding Of Isaac Afterbirth Daily Challenge | 320,292 | Let's Play |
| 68. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 195 [Firework] | 319,930 | Let's Play |
| 69. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 666 [Satanic] | 318,329 | Let's Play |
| 70. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 3 [Greedier] | 317,458 | Let's Play |
| 71. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 24 [Ringo] | 316,732 | Let's Play |
| 72. | YuB | PEE YOURSELF CHALLENGE | The Binding Of Isaac Afterbirth Gameplay [Nintendo Switch] | 316,558 | Let's Play |
| 73. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 5 [Confidence] | 316,091 | Let's Play |
| 74. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 13 [V] | 315,558 | Let's Play |
| 75. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 28 [Karate Kid] | 314,755 | Let's Play |
| 76. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 18 [There's Options] | 314,445 | Let's Play |
| 77. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 17 [Resurrection] | 312,217 | Let's Play |
| 78. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 15 [Windu] | 309,078 | Let's Play |
| 79. |  OfficialNerdCubed | Nerd³ Plays... The Binding of Isaac - Afterbirth+ | 308,933 | Let's Play |
| 80. | OneyPlays | Oney Plays The Binding of Issac - Ep 1 - Mayonnaise | 308,647 | Let's Play |
| 81. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 21 [Introducing] | 305,927 | Let's Play |
| 82. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 14 [Cursed] | 305,574 | Let's Play |
| 83. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 12 [Lazarus] | 305,284 | Let's Play |
| 84. | YuB | isaac perfect accuracy challenge (this sucks) | 301,796 | Let's Play |
| 85. | YuB | THE NEW ISAAC GAME IS OUT AKSLJDHFLAKSJDHFLKAS | 299,948 | Let's Play |
| 86. | YuB | POKEMON IN ISAAC?! | The Binding Of Isaac Afterbirth "Pokey Mans" Challenge Gameplay | 298,738 | Let's Play |
| 87. | YuB | CLUTCH ULTRA GREED FIGHT | The Binding Of Isaac Afterbirth Gameplay [Nintendo Switch] | 297,945 | Let's Play |
| 88. | Northernlion | The Binding of Isaac: AFTERBIRTH+ - Northernlion Plays - Episode 4 [Smelted] | 297,553 | Let's Play |
| 89. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 17 [Lost Boys] | 296,496 | Let's Play |
| 90. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 25 [XXXXXXXXL] | 296,259 | Let's Play |
| 91. | Northernlion | The Binding of Isaac: Antibirth - Northernlion Plays - Episode 7 [Jacob] | 295,537 | Let's Play |
| 92. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 22 [Devout] | 290,232 | Let's Play |
| 93. |  ViteC ► Play | АЛЬФА ИМБА ► The Binding of Isaac: Afterbirth+ |86| AlphaBirth, Quarry mods | 286,716 | Let's Play |
| 94. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 25 [Are You Afraid Of The Dark?] | 285,102 | Let's Play |
| 95. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 42 [The Guardian] | 283,215 | Let's Play |
| 96. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 100 [Hard Mode] | 281,595 | Let's Play |
| 97. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 27 [Speed Up] | 281,429 | Let's Play |
| 98. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 74 [Solar System] | 280,940 | Let's Play |
| 99. | Northernlion | The Binding of Isaac: AFTERBIRTH - Let's Play - Episode 14 [The Lamb] | 280,477 | Let's Play |
| 100. | Northernlion | The Binding of Isaac: Rebirth - Let's Play - Episode 75 [Suicide King] | 280,352 | Let's Play |
