Antagonistic Mechanisms of Bacillus methylotrophicus and Bacillus subtilis
0Antagonistic Mechanisms of Bacillus methylotrophicus and Bacillus subtilis: Exploring Cell Wall-Degrading Enzymes and Siderophore Production for Foliar Blight Management in Wheat
Layman Abstract : Wheat crops are often attacked by harmful fungi called Bipolaris sorokiniana and Alternaria triticina, which cause serious leaf diseases and can reduce wheat harvests by up to 85%. Managing these diseases is very important to ensure good wheat production.
This study found that two beneficial bacteria, Bacillus methylotrophicus (P10) and Bacillus subtilis (UP11), are very effective at stopping these harmful fungi from growing. In lab tests, P10 reduced the growth of Alternaria by nearly 78% and Bipolaris by 74%, while UP11 reduced them by 68% and 62%, respectively.
These helpful bacteria work by breaking down the fungal cell walls and releasing natural compounds that block fungal growth. Because of these abilities, P10 and UP11 could be used as natural, eco-friendly alternatives to chemical pesticides to help protect wheat crops.
This research highlights the importance of further developing these bacteria into biopesticide products for farmers.
----------
Original Abstract : Bipolaris sorokiniana and Alternaria triticina are major cereal pathogens causing spot blotch and leaf blight disease in wheat, with yield losses of up to 85%. These play a central role in wheat foliar blight pathogens of wheat so effective management is essential for sustainable production. Bacillus methylotrophicus (P10) and Bacillus subtilis (UP11) are effective biocontrol agents against foliar blight pathogens Alternaria triticina and Bipolaris sorokiniana. Dual culture assays showed significant growth inhibition, with P10 and UP11 reducing pathogen growth by 77.56% and 67.83% for A. triticina and 73.97% and 62.16% for B. sorokiniana. These strains produce cell wall-degrading enzymes (chitinase, protease, β-1,3-glucanase) and siderophores, peaking at 96 hours in co-cultures with pathogens. Antibiosis and direct inhibition are the primary mechanisms by which these strains suppress the mycelial growth of Bipolaris sorokiniana and Alternaria triticina, emphasizing their role as sustainable biocontrol agents in managing wheat diseases. Their potential as biological control agents underscores the need for further investigation as bioactive ingredients in biopesticide formulations.
https://doi.org/10.9734/bpi/rpmab/v9/4076
#Cell_wall_degrading_enzymes #chitinase #β13glucanse #protease #Bacillus_subtilis #Bacillus_methylotrophicus