Bacillus spp with an Antibacterial Activity

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Bacillus spp with an Antibacterial Activity | Chapter 13 | Recent Advances in Biological Research Vol. 2

Bacterial resistance to antibiotics poses a serious challenge to chemotherapy. Indeed, in recent decades, the risk of contracting infectious diseases has increased significantly due to the emergence and spread of multi-drug-resistant bacteria. The whole world is thus confronted with this situation which motivated scientists to find out new active molecules with an antibacterial effect. Therefore, the present investigation aims to isolate and identify microorganisms secreting substances with an antibacterial activity and to partially characterize their extracts. Thus, six bacteria were isolated from soil and water of different biotopes of Fez Morocco having an antibacterial effect against M. smegmatis, M. aurum, Gram-positive and Gram-negative bacteria. Based on biochemical characterization and 16S rDNA sequence analysis, it was revealed that the isolates belong to the genus Bacillus. The antibacterial activity of three of them was fully affected by proteases (pepsin and proteinase K) and heat treatment at 80ºC and 100ºC but it was stable at 4ºC for a month and 37ºC during 3h. The lost of the activity suggests a proteinaceous nature of the bio-active compounds, which might be useful in the development of antibacterial agents after their total purification in further work against bacterial infections.

Biography of author(s)

Dr. Ilham Zahir
Laboratory of Microbial Biotechnology, Department of Biology, Faculty of Sciences and Technical, University Sidi Mohamed Ben Abdellah, BP 2202, Road of Immouzer, Fez, Morocco and Polyvalent Laboratory in Research and Development, Department of Biology, Polydisciplinary Faculty, Sultan Moulay Slimane University, Beni Mellal, Morocco.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/45/217/371-1



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