BIT: 3 components of recombinant DNA technology| rDNA technology |RDT
2Aoa! MY name Is Ehsan and welcome to Biology with Ehsan
In this lecture we started an important topic of biotechnology named as recombinant DNA technology.
Here we discussed the components or tools of recombinant technology.
There are five important components of recombinant DNA technology
1) Gene of interest
2) Restriction endonucleases
3) Cloning vectors
4) DNA LIGASE
5) Expression system
How gene of interest can be obtained?
What is DNA synthesizer machine?
What are molecular scissors?
What are restricte endonuclease enzymes?
How endonuclease enzyme works?
What is staggered cut?
What is palindromic sequence?
What is site of restriction?
What is blunt cut?
What does it mean by 5' sticky ends and 3' sticky ends?
What is the natural defense mechanism of bacteria against phage viruses?
What is cloning vector?
What properties should be in cloning vector?
What is recombinant DNA?
What is molecular glue?
Why DNA ligase is used in recombinant DNA technology?
What is expression system in recombinant DNA technology?
What properties should a host possess in recombinant DNA technology?
Mention some examples of cloning vectors or carriers?
And many such questions are addressed in this lecture.
In first part of the lecture we discussed the components of recombinant DNA technology or tools required for recombinant DNA technology?
The lecture is very important for biology students especially for federal board biology second year students.
Full conceptual lecture on Recombinant DNA technology in urdu
Full conceptual lecture on Recombinant DNA technology in hindi
Recombinant DNA technology by sir Ehsan
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00:00 Introduction to recombinant DNA technology
08:48 Components of recombinant DNA technology
09:23 Gene of interest
22:00 Molecular scissors| restriction endonucleases
51:42 Molecular cloning vector
61:59 DNA ligase|molecular glue
65:27 Expression system
About:__________________________
Name: Ehsanullah
University: Graduated from Quaid e azam university Islamabad, Pakistan
Department: Department of Biotechnology, Quaid e azam university Islamabad
Link for Biosynthesis of Biomolecules playlist:
https://youtube.com/playlist?list=PLxwfHdURnxtIAyTj-sQvp7rzAlVzernrA
Link for DNA playlist:
https://youtube.com/playlist?list=PLxwfHdURnxtKO2ykwXbSER2DhTOtz4V_D
Link for Biology first year chapter 1:
https://youtube.com/playlist?list=PLxwfHdURnxtIfY-CO38aHt6cgzyox-90q
Link for Bioenergetics:
https://youtube.com/playlist?list=PLxwfHdURnxtKBFWDO9ize7U32Y20YBgWU
Link for Water playlist:
https://youtube.com/playlist?list=PLxwfHdURnxtIbsMuxCxjbjO1OmqObo5ro
Link for Light dependent reactions:
https://youtu.be/1t0yUr4lfrA
Link for Calvin cycle:
https://youtu.be/5B61O0bsnp4
Link for Glycolysis:
https://youtu.be/Bn-LvqwkmSw
Link for DNA replication:
https://youtu.be/rtdvcgZzYP4
Link for exam preparation playlist:
https://youtube.com/playlist?list=PLxwfHdURnxtKR1boHIGVz5dr4Gbqdl7og
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@biologywithehsan2