Optimization of Process Parameters for Improved Lipase Production by Hyperthermophilic Bacillus

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Optimization of Process Parameters for Improved Lipase Production by Hyperthermophilic Bacillus sonorensis 4R | Chapter 05 | Advances and Trends in Biotechnology and Genetics Vol. 2

Thermostable lipases isolated from thermophilic bacteria are emerged as industrially and biotechnologically important bio-molecules in recent era of globalization due to their potential to work at high temperatures. Knowledge of growth conditions and nutritional parameters controlling lipase production is necessary to improve its yield and successful development of an industrial bioprocess without increasing the cost of production. We are reporting the individual and interactive effects of process parameters on lipase production by a hyperthermo-alkalophilic strain of Bacillus sonorensis 4R. In the present study, the individual and combined effects of process parameters on lipase production by a hyperthermo-alkalophilic strain of B. sonorensis 4R are studied. Parameters used in this study were incubation period, temperature, initial pH of medium, carbon and nitrogen sources, substrates and metal salts. The isolate showed maximum lipase production after 4 days of incubation at 80°C and pH 8.0 and when growth medium was supplemented with 1% glucose, 1% ammonium sulphate, 100mmol CaSO4 and by using 1% Tween-80 as lipidic substrate. The combined effects of six variables (pH, temperature, substrate concentration, carbon source, nitrogen source and metal salt) studied in 12 experimental sets showed highest lipase production (51.33 U/mL) by B. sonorensis 4R. In a medium design composed with Tween-80 (1%), CaSO4 (100 mmol), glucose (0.5%), ammonium sulphate (1%), pH (7.5) and when incubated at 80°C, 6.88 fold enhancement over control was observed in lipase production. In the present study, lipase production from B. sonorensis 4R has been optimized using individual and combined effects of simple and easily manageable process parameters. This knowledge will be helpful to many industrial processes to obtain improved enzyme productivity.

Author(s) Details

Dr. H. J. Bhosale
DST-FIST Sponsored School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, 431606, India.

S. Z. Uzma
DST-FIST Sponsored School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, 431606, India.

Prof. T. A. Kadam
DST-FIST Sponsored School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, 431606, India.

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