| 1. | ManlyBadassHero | 424,778 | My Big Sister - What a Twist ( ALL ENDINGS ) Manly Let's Play [ 6 ] | 46:50 |
| 2. |  Verociel | 18,716 | My Big Sister | True Ending | Full Playthrough & Gameplay | 2:16:27 |
| 3. |  TheRPGMinx | 18,229 | OUR SISTER HAS BAD TASTE IN MEN | My Big Sister [2LP] | 13:26 |
| 4. |  KlingonGamerYT | 4,475 | My Big Sister - Playthrough #5 (Bad Ending) - Nintendo Switch | 25:59 |
| 5. |  Graeme Games | 3,674 | FINALE | Let's Play My Big Sister - PART 5 | Graeme Games | 47:01 |
| 6. | Neco The Sergal | 3,580 | I JUST TALKED TO YOU! | My Big Sister (Early Access) - [Part 4] | 13:17 |
| 7. | Minky Toys and Dolls | 2,155 | Cooking With My Big Sister! - Helping My Big Sister in The Kitchen / OMG Big Sister is Amazing Chef | 37:12 |
| 8. |  Magpie | 1,983 | Let's Play My Big Sister (Deutsch) | Bonus #14 Mama | 11:08 |
| 9. | NOCK | 624 | Nock Plays | My Big Sister - The Endings [Early Access] | 18:48 |
| 10. |  Syltrea | 600 | MY BIG SISTER ESPAÑOL | Capítulo 2 "Sombria está muerta!?" | 11:53 |
| 11. | Lucy Elli | 514 | Let's Play My Big Sister #07 Sombrias Depression | 27:42 |
| 12. | MabiVsGames2 | 407 | My Big Sister - Pixelated Horror At It's Finest! | My Big Sister Gameplay/Playthrough/Walkthrough | 36:30 |
| 13. | Mirta | 381 | let's play My Big Sister Part 8 - The End | 24:13 |
| 14. | HerrOokami | Keine Angst, er will nur spielen! | 353 | Der beschwerliche Weg heimwärts 🎐 MY BIG SISTER #008 | 21:34 |
| 15. | RPG Elven Trash Vampire | 296 | COW FART MAGIC // My Big Sister #6 | 24:11 |
| 16. | Kiara0Yunochida | 238 | Let's Play My Big Sister- Blind- Part 9 | 43:19 |
| 17. | Flamez Plays | 234 | My Big Sister [Part 8] | Out of the Loop (Finale, Multiple Endings) - Lets Play My Big Sister | 51:48 |
| 18. | Time2Die731 | 179 | Let's Play My Big Sister (Blind) Part 7 | 30:37 |
| 19. | Longplay Legends | 174 | My Big Sister: Remastered - Full Game Longplay (w/ True Ending) HD | 3:20:32 |
| 20. | digidv85 | 157 | My Big Sister Part 3. Getting transport. (New Game Blind) | 30:27 |
| 21. | LimitedGameNews | 120 | My Big Sister - PlayStation Vita - Trailer - Retail [EastAsiaSoft] [SOLD OUT - Read description!] | 1:33 |
| 22. | Galvatrons | 112 | Unboxing ~ My Big Sister + Just Ignore Them Limited Edition PS Vita ~ EastAsiaSoft (German) | 5:10 |
| 23. | The Violet Hour - Bilicium | 109 | Is this the END? My Big Sister EPS4 | 43:33 |
| 24. | Video Game Wasteland | 108 | Indie Inquiry #9: My Big Sister | Cut short by a huge a cliffhanger! | Gameplay and Impressions | 15:23 |
| 25. |  Sarah Kimochiii | 103 | My Big Sister, let's play VF | 3:36:20 |
| 26. | Famed Killjoy | 99 | Damn Good Noodles ~ 1 ~ Let's Play: My Big Sister | 16:41 |
| 27. |  Vampirschi Gaming | 93 | My Big Sister - Playthrough Part 5 (Both Endings) | 30:17 |
| 28. |  Simantis | 87 | MY BIG SISTER #8 | Die Schleife muss durchbrochen werden! [ENDE] | LET'S PLAY | 37:16 |
| 29. | piep91 | 77 | My Big Sister - Full Playthrough PS4 | 2:39:12 |
| 30. | Gizley | 75 | Homeward Bound - My Big Sister [04] | 27:37 |
| 31. | ChaosLilithKaede | 75 | My Big Sister Let's Play Part 6 ~ Finale w/Credits | 44:59 |
| 32. | SeiyaTV | 59 | AUFGEWACHT aus dem ewigen ALPTRAUM | My Big Sister #08 ENDE | 31:22 |
| 33. | Durinde Plays | 45 | MY SISTER IS A GHOST! - My Big Sister - Let's Play Episode 4 | 12:09 |
| 34. | GamingTrend | 39 | My Big Sister - Let's Play - Switch [Gaming Trend] | 21:42 |
| 35. |  DS Playthroughs | 32 | "My Big Sister" - Full Horror Game Walkthrough (True Ending - No Commentary) | 3:17:54 |
| 36. | VidGamiacUnlocked | 30 | My Big Sister - Asking for help? | 0:44 |
| 37. |  Muso | 29 | Succede DI TUTTO. | My Big Sister Playthrough ITA - Parte 2「FINALE」 | 3:02:14 |
| 38. | Boettius Maximus | 23 | My Big Sister Asking for help? Trophy in the chapter Taken | 1:12 |
| 39. | JackyLP | 23 | My Big Sister [GER/LP] #12 (Gutes + Böses Ende) | 26:03 |
| 40. | AzurKane | 19 | My Big Sister 🏆 All Trophy Pops + Platinum PS4/Vita | 7:23 |
| 41. | belmont gr | 18 | My Big Sister Complete Playthrough / Trophy Guide | 2:06:03 |
| 42. | A.F.K Alles.Furchtbar.Kommentiert | 16 | Das Schleifenparadoxon (Ende) - My Big Sister - #12 - Alex | 17:32 |
| 43. |  hepc85 | 15 | Let's play My Big Sister | 2:01:54 |
| 44. |  TheAnthonyNL | 12 | Let's Play: My Big Sister | 42:46 |
| 45. | Jibsie | 9 | Let's Play My Big Sister Part 2 (FINALE) | 1:40:09 |
| 46. |  Zachary Dauria (CLICKERLICKER19) | 5 | My Big Sister - Sister?... You're Scaring Me! - Full 100% Playthrough (PLATINUM #462) | 4:07:14 |
| 47. | ojakuela YT | 5 | My big sister - 100% Platinum trophy Achievement & all ends #️⃣0️⃣1️⃣6️⃣ - playstation 4 | 4:30:21 |
| 48. | XiscoT | 4 | Gameplay en PlayStation 4 de My Big Sister | 47:36 |
| 49. | AtticusMJ Plays | 2 | My Big Sister 02 | 1:09:40 |
| 50. | Wh0evenisthisguy | 1 | Some Guy Helps His Sister: My Big Sister (Part 3) | 1:37:36 |
| 51. | Weeaboo Baguette | 1 | Let's play in japanese: Unfortunate Ange - 08 - Siscon S | 34:59 |
