| 1. |  FallenShogun Gaming | 12:17:25 | PROTOLIFE NG+ | End | Hard Mode Campaign | Protolife New Game + Campaign | 30:38 |
| 2. |  Daymian play | 10:20:18 | Protolife - s3 | 4:19:46 |
| 3. | shirlierox | 9:09:09 | Will this game make me rage still ?? Protolife revisted in 2023 | 14:30 |
| 4. |  ИгРоМаНиЯ - Игровые Похождения | 6:55:47 | Неожиданный конец / #12 / Protolife | 40:21 |
| 5. | Друзья по прохождению | 6:22:47 | Protolife №13 ФИНАЛ | 46:30 |
| 6. | Maniac_91 | 6:19:02 | Protolife [13]. Финал | 33:28 |
| 7. |  Zones Multiversum | 5:31:14 | Protolife - E16 - The Core [FINAL] | 29:51 |
| 8. |  Caleb Simpson | 4:43:21 | Protolife Walkthrough (Original Campaign) 5 Final Mission - The Core, Experiments | 15:07 |
| 9. | INDIE | 4:05:42 | Protolife №12 | Неплохая пушка... | 26:15 |
| 10. |  Games Mikura | 3:37:05 | #25 Protolife focus | 16:47 |
| 11. |  Wolfens Gaming | 3:25:53 | 【Protolife // 原型生命】創意血尿塔防... 破台啦! | 3:25:53 |
| 12. | iamarugin | 2:15:38 | Protolife: Acid Trip Walkthrough | 13:20 |
| 13. | TLG | 2:11:58 | Protolife. Part 6. TimeLapse Game | 17:55 |
| 14. | Wanderbots | 2:08:32 | I Can't Hold Back The Tide Of Goop! - Protolife | 55:12 |
| 15. | Nikitaro | 1:34:36 | Protolife - [Прохождение #3 - Здоровая опухоль! | 34:10 |
| 16. | Hiki - Plays | 1:15:29 | Protolife - первый заход | 1:15:29 |
| 17. | The Silver Knight | 1:08:24 | ProtoLife Part #2 - #letsplay | 51:43 |
| 18. | Latreis Modding Adventures | 55:22 | Launching ROCKETS from afar at HUGE NEW ENEMIES! Protolife Part 11 | 14:41 |
| 19. | TheBloodSamurai | 47:32 | Fighting Against Endless Infection in This Tower Defense Roguelite Game - Protolife | 47:32 |
| 20. | Inco-opatibleLP | 42:40 | Andrew Shows: ProtoLife [Ep2] | 22:17 |
| 21. | HiroTeaShi | 42:31 | Protolife - Acid Trip NG+ | 26:25 |
| 22. | Dad's Gaming Addiction | 39:29 | DGA Plays: Protolife (Ep. 2 - Gameplay / Let's Play) | 15:17 |
| 23. | Frazzz | 35:51 | Protolife Gameplay - Smash Look! | 35:51 |
| 24. |  Mr Chaser | 35:07 | Protolife - СТРОЙ ЧТОБЫ ВЫЖИТЬ | 35:07 |
| 25. | Valoryouadiu | 31:14 | Being the cell defence system😁☜(ﾟヮﾟ☜)!!!(Protolife) | 31:14 |
| 26. | Mad Play | 30:14 | Protolife ● КУРСОР - ГЛАВНЫЙ ГЕРОЙ ИГРЫ ● ПОГЛЯДИМ ► | 30:14 |
| 27. | GoMenXGames | 28:23 | [Protolife] Очищаем планету #1 | 28:23 |
| 28. | Hultuti | 15:05 | ブロックの配置で防衛網を構築してエイリアンと戦うタワーディフェンスゲーム【Protolife 実況】 | 15:05 |
| 29. | ХайДеш | 14:36 | СМЕРТЕЛЬНАЯ ИНФЕКЦИЯ | Protolife | 14:36 |
| 30. | astray3k | 13:39 | Protolife Gameplay | 13:39 |
| 31. |  PLAYER 7 | 12:54 | LIMPE O PLANETA GAGARIN-5 DAS MINHOCAS GIGANTES | 12:54 |
| 32. |  BeyKaplan | 12:34 | " BAKALIM BAKALIM/ İLK BAKIŞ " - Protolife Türkçe Oynanış | 12:34 |
| 33. | Badin Q3 | 7:53 | Protolife Gameplay - Full Mission Walkthrough | 7:53 |
| 34. |  TheBlueDragon | 5:18 | Protolife Gameplay (PC) | 5:18 |
| 35. |  ألونزو إنريكي ڨونزالوس دي ماركو لا بامبوزا | 4:58 | Protolife - PC Gameplay | 4:58 |
| 36. | AllGamesWorldHd | 4:25 | Protolife Gameplay (PC game) | 4:25 |
| 37. | Monorisu | 3:01 | This is... ProtoLife | 3:01 |
| 38. | CyberLittleVirus Cyberpunk | 2:19 | Protolife | 2:19 |
| 39. | Рус К. | 2:18 | Ludum Dare 38 - Protolife by Volcanic Giraffe | 2:18 |
