| 1. |  INDIE | 161,100 | Protolife №12 | Неплохая пушка... | 26:15 |
| 2. |  Wanderbots | 51,843 | I Can't Hold Back The Tide Of Goop! - Protolife | 55:12 |
| 3. |  shirlierox | 36,259 | Will this game make me rage still ?? Protolife revisted in 2023 | 14:30 |
| 4. | FallenShogun Gaming | 25,539 | PROTOLIFE NG+ | End | Hard Mode Campaign | Protolife New Game + Campaign | 30:38 |
| 5. | iamarugin | 6,801 | Protolife: Acid Trip Walkthrough | 13:20 |
| 6. | Nikitaro | 2,412 | Protolife - [Прохождение #3 - Здоровая опухоль! | 34:10 |
| 7. |  Wolfens Gaming | 2,146 | 【Protolife // 原型生命】創意血尿塔防... 破台啦! | 3:25:53 |
| 8. | Soul Play | 2,083 | 🐍Protolife [ПЕРВЫЙ ВЗГЛЯД] и обзор геймплея, начало прохождения (tower defense) | 43:09 |
| 9. | Dad's Gaming Addiction | 1,848 | DGA Plays: Protolife (Ep. 2 - Gameplay / Let's Play) | 15:17 |
| 10. | Caleb Simpson | 1,712 | Protolife Walkthrough (Original Campaign) 5 Final Mission - The Core, Experiments | 15:07 |
| 11. |  Games Mikura | 1,663 | #25 Protolife focus | 16:47 |
| 12. | ИгРоМаНиЯ - Игровые Похождения | 1,560 | Неожиданный конец / #12 / Protolife | 40:21 |
| 13. | AllGamesWorldHd | 1,524 | Protolife Gameplay (PC game) | 4:25 |
| 14. | Рус К. | 879 | Ludum Dare 38 - Protolife by Volcanic Giraffe | 2:18 |
| 15. |  Mr Chaser | 854 | Protolife - СТРОЙ ЧТОБЫ ВЫЖИТЬ | 35:07 |
| 16. | Frazzz | 634 | Protolife Gameplay - Smash Look! | 35:51 |
| 17. | Hultuti | 611 | ブロックの配置で防衛網を構築してエイリアンと戦うタワーディフェンスゲーム【Protolife 実況】 | 15:05 |
| 18. |  TheBlueDragon | 539 | Protolife Gameplay (PC) | 5:18 |
| 19. |  Zones Multiversum | 532 | Protolife - E16 - The Core [FINAL] | 29:51 |
| 20. | ХайДеш | 528 | СМЕРТЕЛЬНАЯ ИНФЕКЦИЯ | Protolife | 14:36 |
| 21. |  BeyKaplan | 494 | " BAKALIM BAKALIM/ İLK BAKIŞ " - Protolife Türkçe Oynanış | 12:34 |
| 22. | Monorisu | 436 | This is... ProtoLife | 3:01 |
| 23. | HiroTeaShi | 380 | Protolife - Acid Trip NG+ | 26:25 |
| 24. | TLG | 276 | Protolife. Part 6. TimeLapse Game | 17:55 |
| 25. | Друзья по прохождению | 259 | Protolife №13 ФИНАЛ | 46:30 |
| 26. | Latreis Modding Adventures | 136 | Launching ROCKETS from afar at HUGE NEW ENEMIES! Protolife Part 11 | 14:41 |
| 27. | Maniac_91 | 134 | Protolife [13]. Финал | 33:28 |
| 28. |  Daymian play | 126 | Protolife - s3 | 4:19:46 |
| 29. | Mad Play | 126 | Protolife ● КУРСОР - ГЛАВНЫЙ ГЕРОЙ ИГРЫ ● ПОГЛЯДИМ ► | 30:14 |
| 30. | TheBloodSamurai | 113 | Fighting Against Endless Infection in This Tower Defense Roguelite Game - Protolife | 47:32 |
| 31. | GoMenXGames | 38 | [Protolife] Очищаем планету #1 | 28:23 |
| 32. | Badin Q3 | 31 | Protolife Gameplay - Full Mission Walkthrough | 7:53 |
| 33. | The Silver Knight | 31 | ProtoLife Part #2 - #letsplay | 51:43 |
| 34. | astray3k | 23 | Protolife Gameplay | 13:39 |
| 35. | Valoryouadiu | 15 | Being the cell defence system😁☜(ﾟヮﾟ☜)!!!(Protolife) | 31:14 |
| 36. | Hiki - Plays | 8 | Protolife - первый заход | 1:15:29 |
| 37. | CyberLittleVirus Cyberpunk | 8 | Protolife | 2:19 |
| 38. |  ألونزو إنريكي ڨونزالوس دي ماركو لا بامبوزا | 6 | Protolife - PC Gameplay | 4:58 |
| 39. | Inco-opatibleLP | 5 | Andrew Shows: ProtoLife [Ep2] | 22:17 |
| 40. |  PLAYER 7 | 1 | LIMPE O PLANETA GAGARIN-5 DAS MINHOCAS GIGANTES | 12:54 |
