| 1. |  39daph vods | 232,735 | 39daph Plays Puzzle Agent | 4:13:25 |
| 2. | Fuse4Gaming | 2,728 | September 2011 (Puzzle Agent) Contest WINNER! (UPDATE) | 1:01 |
| 3. | rinimt | 858 | Puzzle Agent All Puzzles Speedrun - 34:33 (Current PB) | 42:51 |
| 4. | owlsighs | 371 | [5][FINAL] Puzzle Agent [Owl Playthrough] | 23:00 |
| 5. | PiranhaCupcake | 134 | Puzzle Agent [Ending] | 15:06 |
| 6. | Namoochi | 109 | 🔴EN VTUBER| Puzzle Agent pt.2 and Among Us with viewers! | 3:35:19 |
| 7. | GXP Gaming | 104 | Gnomes Scare Me Now || Puzzle Agent FINALE | 8:33 |
| 8. | ShifterChaos | 100 | Lets Play Puzzle Agent: The Gnomes Made Me Do It (9/9) | 14:43 |
| 9. | Grey Wuskerfluff Ch. | 70 | Puzzle Agent 2: Getting that Hot Dish. 5/6 | 27:16 |
| 10. | Una Yamakuma 山熊ウナ Ch. | 66 | [Puzzle Agent] Here we go again, PUZZLES FEED ME!!! [Vtuber] | 4:18:22 |
| 11. | I Am Heroic | 56 | HE.....IS STEVE | Puzzle Agent | Detective Time | 2 | 0:00 |
| 12. | FinalCut | 41 | Puzzle Agent • Part 5 • ENDING | 40:26 |
| 13. | cowboyotis | 33 | Let's Stream: Puzzle Agent | 2:37:11 |
| 14. | RoflLobster | 24 | Day 563: It was a tragic accident | 1:13:43 |
| 15. | That Imp Girl | 16 | Puzzle Agent Ending! | 2:37:36 |
| 16. | Frog Productions | 14 | Puzzling the Agent | 27:06 |
| 17. | Natal Arts | 10 | Natal Plays Nelson Tethers Puzzle Agent 1 | 3:34:29 |
| 18. | CatGirl George | 10 | Puzzle Agent - On Air! | 2:01:10 |
| 19. | Jon Renish | 8 | Steam Cleaning - Puzzle Agent | 42:17 |
| 20. | Natal Plays Games | 2 | Natal Plays Nelson Tethers Puzzle Agent 1 | 3:34:29 |
| 21. | Game-A-Dayer | 1 | Day 374 - Puzzle Agent | 1:13:16 |
| 22. | 420 GAMING | 1 | 420 GAMING - Nelson Tethers: Puzzle Agent - With the wife | 53:34 |
