| 1. |  jacksepticeye | 6,477,358 | FRIENDS TILL THE END | Riddle Transfer 2 (Series Finale) | 39:18 |
| 2. | Nogla & Lui Gaming | 185,666 | 0 HOURS PLAYED with 200 HOURS PLAYED! | Binding of Isaac Online! | 54:06 |
| 3. | Nogla | 131,397 | Binding of Isaac co-op is the most toxic experience ever | 12:26 |
| 4. | RockLeeSmile | 82,692 | Northernlion Live Super Show [4/11/18] | 3:09:11 |
| 5. | Niall Stenson | 44,293 | Penance/Cellar Theme - The Binding Of Isaac Guitar Cover [60FPS] | 2:27 |
| 6. | The Lucky Roll | 11,574 | The Binding of Isaac: Four Souls - Card game - Review | 9:04 |
| 7. | ForgottenGem | 2,794 | LazyMattman Timeeeee #gaming #streamer #thebindingofisaac #lazymattman | 0:00 |
| 8. | imWhiskerZ | 1,075 | 112 lose streak and you | Binding of Isaac Gameplay | 0:00 |
| 9. | StreamingWiz | 848 | Bro didn't stand a chance #gaming #gameplay #games #streamingwiz #boi #bindingofisaac | 0:00 |
| 10. | Majin Jamie | 523 | that was terrifying | 1:04 |
| 11. | Scorpios9472 | 333 | THE BINDING OF ISAAC - HERE WE GO AGAIN, CAN WE GET MORE ITEMS | 2:19:28 |
| 12. | Moth Girl Hina | 266 | It Dies [VTUBER] | 2:29:41 |
| 13. | TheChrisD | 213 | The Binding of Isaac - #2 - Eve Goes to Vegas | 1:23:07 |
| 14. | InsideAgameR | 101 | THIS IS YOUR LIFE NOW! : Is this my destiny in 5 mins! lets walk through my life together! | 10:44 |
| 15. | Blue Plushtrap | 27 | So I suck at playing The Binding of Isaac | 31:26 |
| 16. | ForgottenGemExtras | 22 | The Binding Of Isaac #48 - Keeper Farming | 1:05:47 |
| 17. | Snodge_ | 12 | The Binding of Isaac - 1st Look - [1] | 2:12:57 |
| 18. | Ishmael | 10 | The binding of Isaac #1 | 6:32 |
| 19. | iFightMice | 8 | VOD 14/6/2024 Elden Ring and The Binding Of Isaac!! | 4:18:49 |
| 20. | Ramba | 7 | [AFTERBIRTH] Azazel Greed Ep.2 | 21:22 |
| 21. | KickaDan | 7 | BOSS FIGHTS!!: The Binding Of Isaac: Ep2 | 12:10 |
| 22. | SheepAreBaaad | 6 | A Sheep plays The Binding of Isaac | 0:00 |
| 23. | Veleck | 0 | the binding of isaac? i sure hope it does! | !steam !discord !throne | 0:00 |
