In PCR, the optimum temperature for denaturing the DNA fragment is , for annealing is respective...

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In PCR, the optimum temperature for denaturing the DNA fragment is , for annealing is respectively. and for polymerisation is
Choose the option that fills the blanks correctly
(1) \( 74^{\circ} \mathrm{C}, 94^{\circ} \mathrm{C} \) and \( 40^{\circ} \mathrm{C} \)
(2) \( 94^{\circ} \mathrm{C}, 50-60^{\circ} \mathrm{C} \) and \( 72^{\circ} \mathrm{C} \)
(3) \( 80^{\circ} \mathrm{C}, 40^{\circ} \mathrm{C} \) and \( 94^{\circ} \mathrm{C} \)
(4) \( 94^{\circ} \mathrm{C}, 72^{\circ} \mathrm{C} \) and \( 50-60^{\circ} \mathrm{C} \)
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