| 1. | jPlay | jPlay plays Star Trek: Ascendancy Borg Assimilation - EP1 | 13,237 | Let's Play |
| 2. | jPlay | jPlay plays Star Trek: Ascendancy Borg Assimilation - EP2 | 6,123 | Let's Play |
| 3. |  MasterofRoflness | Let's Play Thrawns Revenge 2: Ascendancy Mod as Imperial Remnant Final | 5,739 | Let's Play |
| 4. |  slickerdrips | Ascendancy | Overview | 4,536 | Let's Play |
| 5. | Timpedia | Let's Play SoaSER Thrawns Revenge 2: Ascendancy Mod Ep. 5 | 3,625 | Let's Play |
| 6. | jPlay | jPlay plays Star Trek: Ascendancy Borg Assimiliation - EP4 | 3,586 | Let's Play |
| 7. | Space Game Junkie | Let's Play Ascendancy - Entry 1 - I Play, I Lose, I Play Again! (1/5) | 3,426 | Let's Play |
| 8. | The Brothers Murph | Ascendancy Play Through | The Game Haus | 3,416 | Let's Play |
| 9. | Timpedia | Let's Play SoaSER Thrawns Revenge 2: Ascendancy Mod Ep. 6 | 3,173 | Let's Play |
| 10. | Timpedia | Let's Play SoaSER Thrawns Revenge 2: Ascendancy Mod Ep. 7 | 2,742 | Let's Play |
| 11. | Willy Wonka | Let's Play Ascendancy - Part 1 | 2,508 | Let's Play |
| 12. |  kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 01 | 1,060 | Let's Play |
| 13. | Willy Wonka | Let's Play Ascendancy - Part 2 | 753 | Let's Play |
| 14. |  Corey Loses | Thrawn's Revenge: Ascendancy Let's Play #1: Remnant, Pt 2 | 717 | Let's Play |
| 15. | Space Game Junkie | Let's Play Ascendancy - Entry 2 - War, Huh, Good God (2/5) | 657 | Let's Play |
| 16. | Willy Wonka | Let's Play Ascendancy - Part 3 | 455 | Let's Play |
| 17. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 02 | 336 | Let's Play |
| 18. | Space Game Junkie | Let's Play Ascendancy - Entry 5 - War Whiplash (5/5) | 325 | Let's Play |
| 19. | Willy Wonka | Let's Play Ascendancy - Part 4 | 301 | Let's Play |
| 20. | Space Game Junkie | Let's Play Ascendancy - Entry 3 - Tipping the Scales (3/3) | 289 | Let's Play |
| 21. | Space Game Junkie | Let's Play Ascendancy - Entry 4 - GOD DAMMIT (4/5) | 270 | Let's Play |
| 22. | Willy Wonka | Let's Play Ascendancy - Part 13 | 245 | Let's Play |
| 23. | Willy Wonka | Let's Play Ascendancy - Part 5 | 239 | Let's Play |
| 24. |  Spielemagazin | Lets Play Retro: Ascendancy (PC, 1995) Gameplay | 217 | Let's Play |
| 25. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 03 | 201 | Let's Play |
| 26. | Kato | Let's Play Ascendancy #00 - Promotion video | 188 | Let's Play |
| 27. | Willy Wonka | Let's Play Ascendancy - Part 6 | 160 | Let's Play |
| 28. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 04 | 152 | Let's Play |
| 29. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 20 | 135 | Let's Play |
| 30. | Willy Wonka | Let's Play Ascendancy - Part 9 | 132 | Let's Play |
| 31. | Willy Wonka | Let's Play Ascendancy - Part 7 | 128 | Let's Play |
| 32. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 06 | 126 | Let's Play |
| 33. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 05 | 121 | Let's Play |
| 34. | Willy Wonka | Let's Play Ascendancy - Part 8 | 120 | Let's Play |
| 35. | Willy Wonka | Let's Play Ascendancy - Part 12 | 120 | Let's Play |
| 36. | Unfiltered Gamer | Ascendancy Quest for Dominance: Playthrough | 106 | Let's Play |
| 37. | Willy Wonka | Let's Play Ascendancy - Part 10 | 105 | Let's Play |
| 38. | Willy Wonka | Let's Play Ascendancy - Part 11 | 104 | Let's Play |
| 39. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 07 | 103 | Let's Play |
| 40. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 10 | 92 | Let's Play |
| 41. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 08 | 89 | Let's Play |
| 42. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 11 | 84 | Let's Play |
| 43. | Ogiwon TV | Let's Play: Ascendancy 1995 Part 1 Creating the Galaxy | 84 | Let's Play |
| 44. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 13 | 77 | Let's Play |
| 45. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 09 | 73 | Let's Play |
| 46. | HeatedChaos_ | Let's Play Ms Paint #5 Ascendancy | 68 | Let's Play |
| 47. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 14 | 67 | Let's Play |
| 48. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 15 | 65 | Let's Play |
| 49. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 12 | 61 | Let's Play |
| 50. | Skidddog | Skidd Plays: Ascendancy - Part 2 | 61 | Let's Play |
| 51. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 19 | 57 | Let's Play |
| 52. | Skidddog | Skidd Plays: Ascendancy - Part 4 (FINAL) | 54 | Let's Play |
| 53. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 16 | 53 | Let's Play |
| 54. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 18 | 53 | Let's Play |
| 55. | kebzero | Let's Play Ascendancy (1995) - Chamachies - Episode 17 | 52 | Let's Play |
| 56. | Ogiwon TV | Let's Play: Ascendancy 1995 Part 4 First Combat | 40 | Let's Play |
| 57. | LetsTryGG | Forager's Ascendancy - Let's Play Episode 16: Soaring to Great Heights | 36 | Let's Play |
| 58. | Skidddog | Skidd Plays: Ascendancy - Part 3 | 32 | Let's Play |
