| 1. |  Marz | 1,856,405 | Fighting Nashandra (ENDING) | Dark Souls 2 | Marz | 52:18 |
| 2. | SuperButterBuns | 1,078,926 | DARK SOULS 2 FOR BEGINNERS | 13:44 |
| 3. | Kay Plays | 651,901 | Kay Plays Dark Souls II (NG+), Episode 29 | 38:46 |
| 4. |  Shenpai | 635,637 | The Dark Souls 2 Experience | 42:52 |
| 5. | PressHeartToContinue | 529,935 | Poop - Dark Souls II Livestream | 1:03:57 |
| 6. |  Shizuka Rin Official | 524,163 | 𝐃𝐀𝐑𝐊 𝐒𝐎𝐔𝐋𝐒 𝐥𝐥 | 17: 絶望を焚べよ ～しずりん、ダクソ2の世界へ行くの巻～【静凛/にじさんじ】 | 4:10:47 |
| 7. | LifeByLacey | 488,634 | The Pursuer & The Last Giant Dark Souls 2 - LIVE! | 2:02:41 |
| 8. |  Phenrir Mailoki | 468,176 | SABAKU NO MAIKU DISTRUGGE A MARTELLATE LA STATUA DI ORNSTEIN! | 3:06 |
| 9. | Noel Ch. 白銀ノエル | 403,864 | #05【DARK SOULS Ⅱ】(うｐ主失踪シリーズ)ゲリラチック！脳筋女騎士！毒の妃ミダを倒した先へッ🔥【白銀ノエル/ホロライブ】 | 3:20:40 |
| 10. |  Hazelnuttygames | 355,067 | Secret OP Sir Alonne Move: Dark Souls 2 DLC! - Stream Highlight | 1:01 |
| 11. | 葉山舞鈴 / Ohayama Ch. | 351,817 | #07【DARK SOULS II】クリアまでおわれま・・・はやま！【ダークソウル2/完全初見】【葉山舞鈴/にじさんじ】 | 4:54:25 |
| 12. |  GIRLvsGAME | 331,980 | DARK SOULS 2: SOTFS | TITANITE CHUNK GUIDE [Locations+Farming Tips] | 5:57 |
| 13. |  8 Bites | 285,639 | Dark Souls 2 Scholar of The First Sin Walkthrough - Darklurker Boss Fight | 31:16 |
| 14. | Nyanners | 276,427 | Nyanners Beats the Hardest Dark Souls Boss | 0:42 |
| 15. | バーチャルおばあちゃんねる | 173,864 | 3分でわかるバーチャルおばあちゃんの赤服老婆連続暴行不審火事件【ダークソウル2】 | 3:29 |
| 16. | TheRPGMinx | 159,030 | MINX NEEDS HELP!!! | Dark Souls II - RAGE MINX | 03 | 10:18 |
| 17. |  Lomadiah | 102,044 | Hannah Plays! - Dark Souls 2 | 13:44 |
| 18. | GamerJoob | 67,341 | My First Time Ever Playing Dark Souls 2 | Ending | Finale | Nashandra | Throne of Want | 1:31:23 |
| 19. | 葉加瀬 冬雪 / Hakase Fuyuki | 56,132 | 【DARK SOULS】#2 急襲【にじさんじ/葉加瀬冬雪】 | 3:10:59 |
| 20. | 八重沢なとり / Yaezawa Natori | 32,740 | 【ダークソウル2/DARK SOULSⅡ】朝活！！な、なんかホラーなゾーンに侵入してしまったぞ・・・【ゲーム実況】八重沢なとり VTuber | 3:58:00 |
| 21. | Patra Channel / 周防パトラ | 32,335 | 【DARK SOULS2】ダークソウル2 DLCの世界へ飛びだす！初見プレイで挑む！Very difficult additional content! #10【周防パトラ / ハニスト】 | 4:19:01 |
| 22. | Lavinia | 22,944 | Ep 118 The End! -Let's Play: Darksouls II (Scholar of the First Sin Edition) (Blind) | 25:03 |
| 23. | Liera | 15,661 | FINAL EPISODE! Nashandra, Aldia & Darklurker - Dark Souls 2 Blind - Pt 34 | 1:11:40 |
| 24. | 天川はの/AmakawaHano | 8,316 | #08【ダークソウル2】強い敵目指して突撃!!【Vtuber/天川はの】 | 5:04:36 |
| 25. | ꧁ZOPAR - ゾーパー꧂ | 4,636 | 【4連休ダクソやりまくり生活】『ダークソウル2』編 #12（完）【クゥ #VTuber】 | 1:39:17 |
| 26. | ch0colatemilk | 2,935 | Dark Souls II Death Montage | 16:29 |
| 27. | ApolloIsMyCoPilot | 2,302 | Let's Play! Dark Souls II [blind] - S2 P5 - Forest Of Firebombs! | 23:05 |
| 28. | AviGaming | 2,287 | A Night Playing: Dark Souls II | 14:47 |
| 29. | Zer0Doxy | 2,087 | Doxy Death Count VOD: Dark Souls II [10.01.2014] part 2 | 59:33 |
| 30. | GadgetGirlKylie | 1,803 | DARK SOULS II: Scholar of the First Sin - Dragonrider Boss | 1:08 |
| 31. | marleygrey | 614 | WUT!? #darksouls2 #ds2sotfs #darksouls | 0:30 |
| 32. |  Lexycutable | 465 | First Time in Dark Souls II w/ Sven & Redneck | 10:08 |
| 33. | Svela Prisera | 390 | Explosions are Fun | 0:10 |
| 34. | LadyTenjoin | 269 | Dark Souls II Fail: And They All Fall Down... | 0:16 |
| 35. | Rinリン | 226 | 【Dark Souls II Remastered】Fire Keeper Has Awakened【EN VTuber】 #VirtualLiVE | 26:18 |
| 36. |  VtuberArisa | 118 | Stream Dark Souls II: [ENVtuber] Forest and the mist | 1:48:24 |
| 37. | Franki | 63 | DARK SOULS 2 -- BONFIRE: LIT , HOLLOWFICATION: CURED , DICK: OUT | 31:36 |
| 38. | Celina VODs | 55 | Celebrating the 10 year anniversary of Dark Souls 2 by playing a better game - twitch.tv/celina | 0:00 |
| 39. | Tani | 48 | Dark Souls II [Livestream vom 25.04.2023] | 3:48:55 |
| 40. | KrimsonKitty | 34 | Covetous Demon Victory - Dark Souls 2 | 2:31 |
