| 1. |  GameGrumps | Jamestown: Legend of the Lost Colony - Steam Train | 310,875 | |
| 2. |  Team Double Dragon | Jamestown - Monday Morning - PART #1 | 203,460 | |
| 3. | Team Double Dragon | Jamestown - Chez Sips - PART #2 | 95,845 | |
| 4. | Team Double Dragon | Jamestown - Health Advice - PART #3 | 77,425 | |
| 5. | Team Double Dragon | Jamestown - Rip Roaring 2016 - PART #4 | 67,850 | |
| 6. | Team Double Dragon | Jamestown - Making a Murderer - PART #5 | 62,373 | |
| 7. |  Coisa de Nerd | Live #02 (parte 2) - Jamestown. | 55,172 | |
| 8. | Team Double Dragon | Jamestown - Being Tired - PART #6 | 54,052 | |
| 9. | Team Double Dragon | Jamestown - Movie Quiz - PART #7 | 52,192 | |
| 10. | Team Double Dragon | Jamestown - Backseat Gaming - PART #8 | 47,271 | |
| 11. | Team Double Dragon | Jamestown - The Drop - PART #10 | 44,113 | |
| 12. | Team Double Dragon | Jamestown - Double Barrels - PART #9 | 43,907 | |
| 13. | Team Double Dragon | Jamestown - Drink a Coke - PART #11 | 41,084 | |
| 14. | Team Double Dragon | Jamestown - Bombshell - PART #12 | 39,725 | |
| 15. | Team Double Dragon | Jamestown - Crushes - PART #13 | 39,094 | |
| 16. | Classic Game Room | Classic Game Room - JAMESTOWN review | 34,695 | Review |
| 17. | sophycz | Jamestown [Sophy.cz] | 15,917 | |
| 18. | SwitchUp | Jamestown + Switch Review: Steampunk SHOOT 'EM UP! | 14,357 | Review |
| 19. | Vechs | Keyboard Only Gaming 03 - Jamestown (Review & Commentary)(V044) | 13,098 | Review |
| 20. | Sunder | Sunder - Jamestown: A Fresh Take on Shoot'em-Ups | 12,608 | |
| 21. |  Online Station | รีวิวเหยียบล้าน | Jamestown+ ซื้อคนเดียวเล่นได้ทั้งบ้าน!! | 10,996 | |
| 22. | DukeP00L | Jamestown: Ep.3, Boss Finale | 9,389 | |
| 23. | MegaYoYo | Jamestown+ (Playstaion 4) - All Boss Fights (No Damage) | 9,199 | Let's Play |
| 24. | DukeP00L | Jamestown: Legend Of The Lost Colony Ep.1 | 9,067 | |
| 25. | Gematsu | Jamestown+ - Switch and PC Announce Trailer | 8,700 | |
| 26. | Azurè Sky | JamesTown: Legend Of The Lost Colony P.C Review | 7,907 | Review |
| 27. |  Tales of Lumin - Character Creation & More! | Lumin's Christmas Giveaway - 10 x DotA 2 Beta Keys, WoW Game Time, Jamestown, Dungeons of Dredmor | 7,528 | |
| 28. | Scott Manley | Anachronistic Martians - Scott Plays Jamestown | 7,079 | |
| 29. | DukeP00L | Jamestown: Ep.2 | 6,986 | |
| 30. |  Arek The Dragon | Jamestown (PC) - Final Boss - Conquistador/Heart of Mars [Beam, No Vaunt, Difficulty: Divine] | 6,955 | |
| 31. |  Mudprints | Bullet Heaven HD60 #132 - Jamestown [PC] | 6,255 | |
| 32. | Video Chums 🎮😄 | Jamestown+ Gameplay | PS4 Switch | 6,179 | |
| 33. | Modshoot | PC Gameplay : Jamestown [HD] | 5,421 | |
| 34. | UnofficialSoundtrack | The Lost Temple of Croatoa - Jamestown: Legend of the Lost Colony on Piano | 5,408 | |
| 35. | Defunct Games | Review: Jamestown+ (PlayStation 4) | 5,240 | Let's Play |
| 36. | ONE Media + | Jamestown Plus Gameplay Trailer (PS4) | 5,057 | Preview |
| 37. | CrossKnights | Jamestown - Legend of the Lost Colony Part 1: Accused | 5,001 | |
| 38. |  Indie Club | Jamestown - Gameplay - Tutorial + First Level | 4,707 | Tutorial |
| 39. |  9warbane | Jamestown DLC Overview - Gunpowder, Treason, and Plot | 4,620 | |
| 40. | CGRundertow | CGR Undertow - JAMESTOWN: LEGEND OF THE LOST COLONY review for PC | 4,485 | Review |
| 41. | The Hamster Alliance | Jamestown (1 of 3) | 4,172 | |
| 42. | MrDeriv | Vidéotest - Jamestown (PC) | 3,520 | |
| 43. | Beat That Boss | Jamestown + // All Bosses | 3,492 | |
| 44. | Cannot be Tamed | Jamestown + - Short Games for Busy People (Episode 7) | 3,408 | |
| 45. | Virtual Gaming Library - VGL | Jamestown: Legend of the Lost Colony PC FULL GAME Longplay Gameplay Walkthrough Playthrough VGL | 3,211 | Walkthrough |
| 46. | CGRoverboard | CGRoverboard JAMESTOWN: LEGEND OF THE LOST COLONY for PC Video Game Review | 3,040 | Review |
| 47. | The Hamster Alliance | Jamestown (3 of 3) | 2,967 | |
| 48. |  IndieMag | Indie Review - Jamestown (PC/Mac) | 2,889 | Review |
| 49. | Gamekult | Pipo et Gautoz prennent leur envol sur Jamestown+, la version définitve du shmup de 2011 | 2,597 | |
| 50. | IndieMag | Jamestown Plus | TEST (Playstation 4) | 2,397 | Let's Play |
| 51. | Scribe | Let's Play Jamestown (#1) - The Lost Colony | 2,396 | Let's Play |
| 52. | quidsup | Gaming in Linux: Jamestown Legend of the Lost Colony | 2,389 | |
| 53. |  CON 5 DUROS | DIRECTO: JAMESTOWN+ (INTENTOS DE 1CC A 4 JUGADORES) (2x1cc) | 2,339 | |
| 54. |  PureRuby | Jamestown+ - Chapter 5 - The Lost Temple of Croatoa - Divine Difficulty - No Damage | 2,250 | |
| 55. | Naka Teleeli | Maiden Reactions Finale [5/6] Jamestown | 2,183 | |
| 56. | gNatFreak | Jamestown - Amusing Antics | 2,129 | |
| 57. | karterfreak | Jamestown Chapter 1 - East Frontier: Judgement Difficulty | 2,061 | |
| 58. | GameSpot | Jamestown: Legend of the Lost Colony - Daily Demo (PC) | 2,057 | |
| 59. | The Hamster Alliance | Jamestown (2 of 3) | 1,990 | |
| 60. | Violet Procyon | Steam Winter Objectives: With Your Nose So Bright... -- Jamestown | 1,976 | |
| 61. | Spaz Boys Gaming | "Press X To Not Die" Gameplay, Part 2 I Get Staple Gunned In The Huevos | 1,909 | |
| 62. | aldershot8008 | Jamestown: Impressions (shoot'm up, shmup, Indie Game, Gameplay, and Review) | 1,754 | Review |
| 63. | Thiima | Jamestown - 4 Player - Level 5 (Croatoa) - Legendary | 1,753 | |
| 64. | FranFrikiYT | Jamestown: Legend of the Lost Colony (PC Indie) - ¡Completo! (Gauntlet Mode) | 1,748 | |
| 65. | AllGamesWorldHd | Jamestown Plus Deluxe Pack Gameplay (PC Game) | 1,737 | |
| 66. | Golden Gamer | Exploring The Jamestown Mall 2 Days Before Demolition | 1,613 | |
| 67. | ZurZockerZone | Lets Play KlaZZiker JAMESTOWN - Das LP des Jahres | 1,456 | Let's Play |
| 68. | Czwórka | Co to jest?! Jamestown | 1,338 | |
| 69. | GameX TV | Jamestown - Review Video in limba romana | 1,242 | Review |
| 70. | Link_Z | Jamestown - Gauntlet no miss (Divine difficulty) | 1,234 | |
| 71. |  AlvaLynsis | Jamestown+: Judgement Super Gauntlet 1cc (21,041,155) | 1,233 | |
| 72. | BananaStudio_Life | Jamestown Legend of the Lost Colony gameplay HD | 1,199 | |
| 73. | ChaosD1 | War is Bullet Hell "Jamestown+" Quick Review | Indie Stop Speedpass | 1,168 | Review |
| 74. | 8BE | Farabel for Nintendo Switch - First Impressions | 8 Bit Eric | 8-Bit Eric | 1,067 | First Impressions |
| 75. | IGN Plays | Daemon Plays Jamestown Plus - IGN Plays | 1,059 | Let's Play |
| 76. | BountyHunterSAx | Let's Play - Jamestown (shmup) | 1,024 | Let's Play |
| 77. | RobinLSL | Let's Play Jamestown - 1 - Non-japanese Shmup! | 1,019 | Let's Play |
| 78. | Thiima | Jamestown - 4 Player - Level 3 (The Badlands) - Legendary | 1,001 | |
| 79. | Scribe | Let's Play Jamestown (#2) - Credits Where Credits Are Due | 981 | Let's Play |
| 80. | PureRuby | Jamestown+ - Chapter 1 - War Upon The East Frontier - Divine Difficulty - No Damage | 971 | |
| 81. | Les Joueurs Du Dimanche | Y A TROP DE BOULES !!! - Jamestown | 968 | |
| 82. |  VoodooHeadsTV | Jamestown - Gameplay (pc) | 968 | |
| 83. | Arek The Dragon | Jamestown (PC) - Boss 1 - The Traitor Prince [Beam, No Vaunt, Difficulty: Divine] | 913 | |
| 84. | Rawiioli | Jamestown Rawiioli Firstlook PC German | 902 | First Impressions |
| 85. | CrossKnights | Jamestown - Legend of the Lost Colony Part 2: The Conquistador | 889 | |
| 86. | Ausgamia | Ausgamia Plays Co-op - Jamestown (Part 1 - East Frontier & Dark Sector) | 885 | Let's Play |
| 87. | Drunge | Jamestown, un shmup de folie ! - Videotest FR PC - KanardWC | 873 | |
| 88. | Major Air | NATIONAL COMEDY CENTER TOUR 2022 - Jamestown, NY | 862 | |
| 89. | Scribe | Let's Play Jamestown (FINALE) - Legend of the Hidden Temple | 827 | Let's Play |
| 90. | RobinLSL | Let's Play Jamestown - 2 - From robots to aliens, to robots | 816 | Let's Play |
| 91. | James Allen | Jamestown Legend of the Lost Colony: Level 3, Legendary, Charge Ship | 807 | |
| 92. | PureRuby | Jamestown - Impossible Combo Trophy Guide | 801 | Guide |
| 93. | MGT - Max Games Tech | Jamestown+ Nintendo Switch handheld gameplay | 792 | |
| 94. | edusword | Jamestown: Legend of the Lost Colony [PC Windows] - ALL Clear - 1CC - edusword | 768 | |
| 95. | DeoGenZ Entertainment | Let's Play Jamestown Legend of the Lost Colony- Episode 1 East Frontier | 767 | Let's Play |
| 96. | The 7th Level | Ten Minute Taste: Jamestown+ (PS4) | 756 | |
| 97. | LONEPLAY Classics | JAMESTOWN+ CO-OP 3 Players (STEAM) | 746 | |
| 98. | Highwang | Jamestown, Stage 1- War on the Eastern Front | 733 | |
| 99. | gamezplay | Jamestown Plus top down 4 player retro shooter now on Switch | 730 | |
| 100. | Kord364 | Jamestown: Legend of the Lost Colony (All Bosses) | 724 | |
