| 1. |  fgw | 326,638 | The Room Two - Full Game Playthrough (No Commentary) | 1:46:40 |
| 2. | Zisteau | 209,842 | The Room 2 Full Playthrough (stream archive) | 4:50:10 |
| 3. | Keith Ballard | 108,897 | Let's Play The Room Two Part 5 FINALE - The Laboratory | 46:15 |
| 4. | PeteDorr | 27,436 | Pete Plays: The Room Two (iPad) | 25:22 |
| 5. | StephenPlays | 26,886 | "Tud or Fud" - Split The Room #2 | Stephen + Friends | 16:33 |
| 6. | IndieGamerRetro | 17,459 | SabKnght Plays ~ The Room Two [Chapter 6: The Lab] | 25:42 |
| 7. | TheCoolJalapeno | 8,690 | Let's Play The Room Two Part 7 (FINALE) | WHERE IT ALL BEGAN | 25:51 |
| 8. | Amaz | 4,006 | THE ROOM #1 - Classic Puzzle Game! - Amaz Plays | 52:34 |
| 9. | Merric Gaming | 736 | Let's Play – The Room Two - Episode 5 [Tentacles]: | 35:16 |
| 10. | Table | 548 | Table Plays - The Room 2 - Part 4 Laborious Laboratory | 30:54 |
| 11. | StoryRetold | 420 | The Room Two (PC Game) | Playthrough #4 | 53:20 |
| 12. | The SaD Games | 382 | Let's Play | The Room Two - FINALE - Electric Laser Boogaloo | 32:52 |
| 13. | ThorH13 | 360 | Thor Plays The Room 2 - Part 6 | 11:04 |
| 14. | Midnight Researcher | 316 | The Room Two | Game Playthrough | 1:29:42 |
| 15. | Indie Chris | 273 | Reanimation Lab - Let's Play The Room Two - FINALE - Part 5 | 51:26 |
| 16. | DotSlashFrag | 204 | Wonders of Science — Let's Play The Room Two: Chapter 5 and 6 — Finale | 29:00 |
| 17. | Pandy351 | 184 | Pandy351 Plays The Room Two! Live Stream | 2:20:54 |
| 18. | AwesomeMattG | 181 | Let's Play: The Room 2 (002) | 1:22:54 |
| 19. | Slay With Brandy | 162 | CREEPIEST ESCAPE ROOM!? | "THE ROOM TWO" - Full Playthrough 👻 | 2:38:20 |
| 20. | MasterWolfin | 144 | Battery power ~ The Room Two (end) | 21:53 |
| 21. | Jazzy Waffles | 125 | The Room Two (Full Playthrough) - The Story Continues! | 1:30:52 |
| 22. | VertPlaysGames | 110 | vert plays... The Escaper [02] │ Room Two | 39:19 |
| 23. | Marstead | 100 | ✔️️ Full Blind Playthrough (The Room 2) | 2:35:46 |
| 24. | Diamond Surge | 87 | THANKS FOR 200 SUBS! | Let's Play The Room 2 (FULL GAME) (ENDING) | 4:40:04 |
| 25. | Generasian X | 73 | The Room Two (Apple Arcade Playthrough) Fireproof Studios | 1:04:11 |
| 26. | GamingSugoi | 55 | The Room Two FULL Game Walkthrough / Playthrough - Let's Play (No Commentary) | 1:05:23 |
| 27. | tacoline | 48 | Let's Play The Room Two pt 7 Final | 29:03 |
| 28. | NeoMegaMan | 46 | Let's Play The Room Two FINALE! That Goddamn Laser Room | 43:46 |
| 29. | Achievement Hunter Xeinok | 37 | The Room Two: 100% Achievements Playthrough / Let's Play | 2:03:11 |
| 30. | duffking56 | 26 | Let's Play: The Room 2 - Following AS (Full Game/Longplay) | 2:14:00 |
| 31. | Neopacket | 19 | Lets Play: The Room 2 ! - 40 before 40: Game 6 | 3:33:48 |
| 32. | JohrDasat | 15 | Let's Play the Room 2 Pranked Called by Satan! | 7:56 |
| 33. | Kitathi | 9 | The Mysteries Around the Null Element - The Room 2 - Trapped in a Fruitless Abyss (Full Playthrough) | 1:39:33 |
| 34. | LostInSideQuests | 8 | First Playthrough of The Room Two | 4:09:36 |
| 35. | BobbyBot | 7 | The Room Two | FULL GAME - LONGPLAY | Livestream | 2:54:21 |
| 36. | odinzate | 7 | Zero commentary gameplay: No talking, just gaming. | The Room Two COMPLETE playthrough | 1:24:46 |
| 37. | Helbeard | 4 | The Room Two - Let's Play | 2:31:01 |
| 38. | Shibby King | 3 | Shibby King Plays The Room Two P3 The Finale | 25:03 |
| 39. | kobe_skrobe | 3 | The Room Two - Ep.2 | Help Me Solve These Puzzles, Please (VOD) | 1:35:47 |
| 40. | Angels Beckoning | 1 | Let's Play: The Room 2 Part 3 | 45:20 |
| 41. | Bicker Brothers Gaming | 1 | Franken Puzzle - Robbie Plays The Room 2 - Part 5 End | 41:46 |
