A biologist was experimenting with a protozoan and noticed that the animal's contractile vacuole...

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A biologist was experimenting with a protozoan and noticed that the animal's contractile vacuole stopped working although the other parts of the organism seemed to be in good health and exhibited normal activity. Which of the following experiment was likely to have produced this result?
(A) Changing the \( \mathrm{pH} \) of the medium from \( 7.00-6.50 \)
(B) Cooling of the medium from \( 20-10^{\circ} \mathrm{C} \)
(C) Transferring the protozoan from sea water to pond water
(D) Transferring the protozoan from pond water to sea water
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