Gamedev Toolbox: ImageMagick
Imagemagick is the swiss army knife of image manipulation. Available as a set of command line utilities or as a C library, if you need to do something to an image or a series of images, Imagemagick can probably do it. Completely free, open source and available on pretty much every platform, there is no reason not to have it installed!
The commands used in this video:
Split into tiles
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convert -crop 32x32 RoyalTileset-TileB.png out\tile_%d.png
Turn tiles into a single image
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montage *.png -tile 8x -geometry 32x32 out.png
Set spacing using the -geometry tag
Convert formats
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convert *.jpg +adjoin out%d.gif
Or
mogrify -format jpg -- *.png
Mogrify with no out params modifies the value in place. For example
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mogrify -resize 200% *.png
mogrify -blur 22 *.png
mogrify -border 2 -bordercolor black *.png