In an experiment, the \( E \). coli was grown and in \( { }^{15} \mathrm{NH}_{4} \mathrm{Cl} \) medium, later in \( { }^{14} \mathrm{NH}_{4} \mathrm{Cl} \) medium for three generations. Based on this given information which of the following statements can be drawn as a conclusion?
I. \( { }^{15} \mathrm{~N}^{15} \mathrm{~N},{ }^{15} \mathrm{~N}^{14} \mathrm{~N} \) and \( { }^{14} \mathrm{~N}^{14} \mathrm{~N} \) bands are formed in the \( 0: 7: 1 \) ratio after three generations.
II. The given experiment does not follow semiconservative model of replication.
III. DNA extracted form the culture after second generation have equal amount of \( { }^{14} \mathrm{~N}^{15} \mathrm{~N} \) and \( { }^{15} \mathrm{~N}^{15} \mathrm{~N} \) DNA.
IV. After one generation of growth, all the DNA molecules contain one \( { }^{15} \mathrm{~N} \) DNA heavy strand and one \( { }^{14} \mathrm{~N} \) DNA light strand and thus be of intermediate density.
(1) I, II and III
(2) II and III
(3) Only IV
(4) I, II, III and IV
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