Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes
26Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks | Chapter 05 | Research and Development in Agricultural Sciences Vol. 2
Aims: As optimization of farm animals reproductive performance is a main objective, the present study was, undertaken to investigate the possible protective effect of vitamin C, vitamin E and olive pomace against dizocilpine (MK-801) induced oxidative stress and its resultant alterations on antioxidant status, spermiogram, hormonal, enzyme markers and histomorphology of testes of rabbit bucks during first and second month of the experiment.
Study Design: Laboratory experimental design was performed.
Place and Duration of Study: The study was conducted in the experimental rabbitry of Physiology Department, Faculty of Veterinary Medicine Cairo University during the period from January to May 2014 in accordance with the Chinese guidelines for animal welfare and approved by the animal welfare committee of Animal Science College, Zhejiang University.
Methodology: Thirty- six male New Zealand White rabbits of about 5-months age and average body weight of 2500 g were randomly selected, used for the experimental work one month later. During this month, the rabbits were gradually adapted to natural mating. The experiment lasted for 2 months (length of spermatogenic cycle in rabbit), bucks were housed individually in commercial cages (55×60×34 cm), equipped with automatic drinkers and j-feeders. Clean and fresh water was available all time. The whole rabbitry was well ventilated through both natural windows and electric fans and illuminated to 14:10 light dark cycle through natural and fluorescent lighting. The rabbitry average ambient temperature and relative humidity ranged from 20 to 30ºC and 70 -80%, respectively during summer resting period. Basal and experimental diets were formulated to cover the nutrient requirements of rabbits as recommended in [1]. Diets were subjected to chemical analysis according to [2]. Offered for all animals ad libitum Table 1. The bucks were equally and randomly divided into six groups (6 in each) namely control which injected with 1.0 ml sterile saline intraperitoneal (i.p.), second group injected by dizocilpine (MK-801 purchased from Sigma-Aldrich, Egypt), at dose 0.1 mg/kg. The dose of MK-801 was dissolved in 1.0 ml sterile saline and was injected daily intraperitoneal (i.p.) for 5 consecutive days, third group was injected by dizocilpine and supplemented with “vitamin C” (1 g/L of drinking water), while the forth group was injected by dizocilpine and supplemented with “vitamin E” in drinking water (50 ml/100 L)( the international units of vitamin E contained in 50 ml be mentioned) and those of the fifth group was supplemented with “vitamin C” plus “vitamin E”. All five groups were fed the basal diets, while the sixth group was injected by dizocilpine and supplemented with the experimental diet that contained olive pomace (10%).
1) Samples collection: Semen was collected using artificial vagina [3]. Semen collection was done by using a teaser female and artificial vagina (containing water at 50ºC) that was locally fabricated as described by Herbert and Adejumo [4]. The ejaculate volumes were recorded (using a graduated collection tube) after removal of the gel mass; Semen pH was determined using pH paper 1-14; Mass motility (MM) was determined by placing a drop of semen on a clean, dry, warm slide and examining microscopically using thermostatically controlled hot stage adjusted at 38-40ºC. Mass activity of spermatozoa was scored (0-5) according to the intensity of the moving whirls [5] as follows: 0 = no current, 1 = few slow current, 2 = many moderate waves, 3 = many sweeping waves, 4 = numerous vigorous waves, 5 = numerous rapid and vigorous waves. Individual motility was assessed in semen sample diluted with 2.9% sodium citrate dehydrate solution, spread almost evenly under a glass cover slide and examined microscopically using adjusted hot stage at 38-40ºC.
Author(s) Details
Sohair Y. Saleh
Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Francois A. R. Sawiress
Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Mohamed A. Tony
Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Amin M. Hassanin
Department of Cytology and Histology, Faculty of Veterinary Medicine, University of Sadat City, Meonofya, Egypt.
M. A. Khattab
Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
M. R. Bakeer
Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
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