Sprouting Value Index: A Research Perspective in Artificial Vegetative Propagation in Tree Species
0Sprouting Value Index: A Research Perspective in Artificial Vegetative Propagation in Tree Species
Layman's Abstract: The genus Brassica includes important crops like canola, and this study looked at how different cultivars of Brassica napus (canola) regenerate from plant tissue. Researchers focused on finding the best conditions to grow callus (plant cells that can grow into new plants) from cotyledon (seed leaves) and hypocotyl (stem) explants. They found that a specific combination of growth regulators helped the best callus formation, especially in one cultivar called Star. To improve plant regeneration, they also used a technique where explants were placed in a growth regulator-free medium for seven days before adding the regulators back in. This method was successful, with most explants developing shoots and some even forming roots on the same medium. The results showed that hypocotyl explants were more successful than cotyledonary explants in forming shoots. In conclusion, this study suggests using hypocotyl explants, particularly from the Westar cultivar, for gene transformation experiments in canola.
#BrassicaNapus #CanolaCultivars #PlantRegeneration #TissueCulture #GeneTransformation #BrassicaResearch #CropScience #PlantResearch #CanolaRegeneration #AgriculturalScience #HypocotylExplants #TissueCultureTechniques #PlantGrowthRegulators
View Book:- https://stm.bookpi.org/RPBS-V1/article/view/17824
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