| 1. | Northernlion | 312,860 | 1001 Spikes - Northernlion Plays - Episode 6 | 26:39 |
| 2. |  bisnap | 157,334 | Bisnap Plays 1001 Spikes - Episode 15 | 48:26 |
| 3. | BaerTaffy | 74,002 | Baer Plays 1001 Spikes (Pt. 12) - COOLEST BOSS EVER | 42:58 |
| 4. | Super Beard Bros | 19,775 | 1001 Spikes | Let's Play Ep. 4 | Super Beard Bros. | 48:33 |
| 5. |  zugzuj | 13,947 | Lets Play 1001 Spikes Vol.30 (German) [PS4/HD/Complete] | 14:20 |
| 6. | ExandShadow | 13,678 | Let's Play 1001 Spikes - Part 8 (BLIND) | 15:20 |
| 7. | RedPandaGamer | 6,317 | Let's Play 1001 Spikes! (PC) - Part 3 - YOU ARE DEAD | 28:25 |
| 8. | n00b22222 | 5,145 | Let's Play 1001 Spikes Part 17. Final. | 35:37 |
| 9. |  Gar | 4,945 | 1001 Spikes (Commentary) Part 7: Chilling Conclusion | 46:42 |
| 10. | RageSelect | 4,517 | Indie Friday - 1001 Spikes | 34:10 |
| 11. | My Life in Gaming | 3,481 | How to Beat #11 - 1001 Spikes / MY LIFE IN GAMING | 11:59 |
| 12. | SolarLune | 2,283 | Let's Play 1001 Spikes #5: I Was Supposed To Push The Block | 17:13 |
| 13. | Eckyman | 2,258 | Ecky Plays 1001 Spikes | Episode 04 | 34:36 |
| 14. | Sunburned Albino | 2,090 | 1001 Spikes - Sunburned Albino Plays - El Testigo Character Run | 47:40 |
| 15. | GameFront | 1,703 | The GameFront Gauntlet - 1001 Spikes - Round #3 | 26:19 |
| 16. | MFSGamePlay | 1,117 | 1001 Spikes - Nintendo (Switch) - Gameplay | 31:58 |
| 17. | Gameplay and Talk | 1,104 | 1001 Spikes Full Playthrough (Ukampa, XBOX ONE) | Let's Play #164 - Precision Platforming Excellence | 1:30:23 |
| 18. |  AyorSaeba | 867 | [Let's Play] 1001 Spikes (PC)(Part 4/4) | 1:12:01 |
| 19. | Visual Novelty | 545 | Let's Play 1001 Spikes! Part 5: VOSTOK (Uncut) | 55:39 |
| 20. |  Maxquaza | 523 | New PlayStation Vita Game Releasing Soon! (1001 Spikes) | 2:49 |
| 21. | Tobes Plays! | 321 | Lets Play 1001 Spikes :: Crushed Controllers And Dreams (Ep 6) | 23:07 |
| 22. | IndieGeek | 258 | IndieGeek Plays: 1001 Spikes Ep 10 [2nd Time's The Charm] | 20:30 |
| 23. | FCRacer | 245 | FCRacer Plays 1001 Spikes: Episode 16: End of the line. | 14:04 |
| 24. | Shawn Bonnett | 192 | Let's Play - 1001 Spikes - Day 32 | 11:35 |
| 25. |  Jennyverse | 187 | STEAMARAMA 2021 (#0-9): 1001 Spikes (Let's Play) | 34:04 |
| 26. | BrianD0313 | 106 | Let's Play 1001 Spikes #007 - Finale (720p) | 1:20:56 |
| 27. | Because Games | 106 | THIS IS HOW I WILL DIE | let's Play 1001 Spikes as a drinking game | 5:01 |
| 28. | Kyle Satwon | 104 | 1001 Spikes: Tower of Nannar (Full Playthrough) | 22:55 |
| 29. | Hobo Ryan | 103 | Hobo Ryan Plays 1001 | 18:16 |
| 30. | Geeks and Nerds | 82 | Pablo Plays - 1001 Spikes (STEAM) - THE GOLDEN VASE - PART 22 | 30:24 |
| 31. |  DS Playthroughs | 62 | "1001 Spikes" - Tower of Nannar - Tower 2 (Hasis) | 16:33 |
| 32. | Noaksey | 56 | Noaksey & XaosWolf in Aban Hawkins & the 1001 Spikes - #2 | 25:02 |
| 33. | Doodrun | 53 | 1001 Spikes #5 | F***ING GOLDEN SAW | 26:19 |
| 34. | GabyOG | 38 | 1001 Spikes - Blind Playthrough | 3:14:22 |
| 35. | headbanger1186 | 32 | Let's Play: 1001 Spikes | 45:45 |
| 36. | AveBatTV | 27 | Let's play 1001 Spikes 1080p with commentary by DinXy | 10:14 |
| 37. | Rods Kaden | 26 | Rods Plays 1001 Spikes (Part 6) - INDIE SUMMER! | 17:43 |
| 38. | AirezRize | 15 | Is this the End?! Let's Play - 1001 Spikes; The Lost Levels P2 | 11:18 |
| 39. | Jolan Live | 10 | [Let's Play Fr] 1001 Spikes - 01 - Soac & Jolan | 1:27:14 |
| 40. | Draverd | 8 | Lets Play 1001 Spikes Part 3 | 44:59 |
| 41. |  Primal Gaming | 0 | Primal Plays - 1001 Spikes | 32:09 |
