| 1. |  IGN | 14,393 | Daemon Plays Jamestown Plus - IGN Plays | 13:42 |
| 2. | MegaYoYo | 9,199 | Jamestown+ (Playstaion 4) - All Boss Fights (No Damage) | 14:19 |
| 3. | Defunct Games | 5,240 | Review: Jamestown+ (PlayStation 4) | 4:32 |
| 4. | Scribe | 4,204 | Let's Play Jamestown (FINALE) - Legend of the Hidden Temple | 18:47 |
| 5. | Kybernauts | 4,191 | Let's Play Česky - JamesTown - Díl 1 | 21:40 |
| 6. |  RobinLSL | 3,363 | Let's Play Jamestown - 5 - The too long video | 27:15 |
| 7. | DeoGenZ Entertainment | 1,590 | Let's Play Jamestown Legend of the Lost Colony- Episode 5 Finalee | 14:03 |
| 8. | ZurZockerZone | 1,456 | Lets Play KlaZZiker JAMESTOWN - Das LP des Jahres | 41:30 |
| 9. | Yessik Ziiiq | 1,451 | Let's Play Jamestown - Part 8 - The Conquistador | 41:24 |
| 10. | IGN Plays | 1,059 | Daemon Plays Jamestown Plus - IGN Plays | 13:42 |
| 11. | BountyHunterSAx | 1,024 | Let's Play - Jamestown (shmup) | 3:35 |
| 12. |  Ausgamia | 979 | Ausgamia Plays Co-op - Jamestown (Part 3 FINALE - Croatoa) | 9:40 |
| 13. |  ColeNL112 | 618 | Let's Play Jamestown: Legend of the Lost Colony [Final]: Conquistador's Last Stand in Croatoa | 23:12 |
| 14. | Catalysts Gaming | 400 | Legend of the Many Deaths! Let's Play Jamestown Co-Op Pt. 2 | 23:50 |
| 15. | Omnifas | 381 | Let's Play Jamestown: Legend of the Lost Colony The End | 27:12 |
| 16. | Backlogged Games | 356 | Jamestown + (Plus) on PS4 – Gameplay Part 1 │ Backlogged Games | 11:03 |
| 17. | Corrupt | 350 | Jamestown Plus Gameplay - NEW Retro Shoot Em Up PC Game | 9:24 |
| 18. | Phlip | 325 | Let's Play Jamestown - 5 - (Croatoa) | 23:51 |
| 19. | PS5 & Stuff Guy | 287 | Let's Play Jamestown Quickly LOL AMAZING Gameplay BANG PS4 XBOX | 3:51 |
| 20. | Five Points Games | 256 | Let's Play! Jamestown: I Did It!!! | 19:30 |
| 21. | Retro Gamer Ranting | 228 | Twitch and Friends Play: Jamestown (Ep 4) "No end in sight" | 5:11 |
| 22. | OrganizedRemains | 218 | Jamestown Let's Play With Organized Remains | 17:56 |
| 23. | Battle Geek Plus - Press Start To AWESOME! | 214 | Jamestown+ Let's Play (PS4) - Battle Geek Plus: We Play Games! | 10:43 |
| 24. | AmitDabydeen | 205 | Jamestown - Legend of the Lost Colony PC 720P HD Playthrough - CHAPTER 5 - FINAL | 18:55 |
| 25. | Drops99full | 175 | Zagrajmy w Jamestown #1 Chapter 1-3 [Let`s Play] | 20:48 |
| 26. | Joey Zero | 164 | Jamestown: Legend of the Lost Colony [pt 4 - Joey Zero] | 5:37 |
| 27. |  UltimateBIM | 157 | JAMESTOWN [19] - Sperrfeuer! Barge Death Bonus [GERMAN/DEUTSCH OV LET'S PLAY] | 2:01 |
| 28. |  Reggiez Corner - Gaming & Guides | 150 | Jamestown - Let's Play - Chapter 5 "The Lost Temple" - With Commentaries | 18:03 |
| 29. | BlueMaxima's Remote Play Channel | 146 | Jamestown+ PlayStation 4 Remote Play Vita Gameplay | 5:31 |
| 30. | NeosForme | 139 | Jamestown - Neos Plays | 20:51 |
| 31. | The Restart Collective | 134 | 1001 Spikes - Indie Restart - Let's Play PS4! | 54:03 |
| 32. | greasyjoystick | 131 | Jamestown - Playthrough Part 4 | 13:53 |
| 33. | LimitedGameNews | 115 | Jamestown+ - PlayStation 4 - Trailer - Physical [Limited Run Games] | 1:05 |
| 34. | The Kyuutie Creator (Apocalypse Nine) | 83 | Jamestown Playthrough - Part 5 The Finale | 18:57 |
| 35. | The PapaKarcher Experience | 80 | Karcher and friends Lets Play Jamestown | 25:03 |
| 36. | Squall Ranger | 78 | Let's Play Jamestown Plus | 29:39 |
| 37. | Lloydarts | 77 | Checking Out | Jamestown+ | 20:32 |
| 38. | Steve's Super-Fun-Time | 74 | 「1」 ★ Laute(r) Explosionen - Let's Play Jamestown [SuperFunTime] | 17:43 |
| 39. | SiouxCity Journal | 71 | WATCH NOW: Viterbo plays Jamestown in NAIA pool play volleyball action | 1:11 |
| 40. | CodeChannel | 68 | Jamestown -- Co-op Playthrough (2 of 3) Jan 20th 2015 | 22:17 |
| 41. | Gamenetreviews | 61 | Jamestown Legend of the Lost Colony: Lets Play | 10:10 |
| 42. | Sam Wayzeer | 58 | Let's Play - Jamestown ep 5 - Legendary action | 10:14 |
| 43. | Ayato -666 | 53 | Lets Play Jamestown Plus Prt 2 | 27:38 |
| 44. | neXGam | 48 | Jamestown+ Longplay (Playstation 4) | 46:36 |
| 45. | RealGenericDemon | 46 | Jamestown - Let's Play Together featuring RealGenericWolf - Prisoner of the Badlands - PS4 | 9:40 |
| 46. | SGBusby1984 | 44 | Gunbird 2 (PS4) Fun Run - Tavia Playthrough | 15:39 |
| 47. | coregamertreff | 40 | Jamestown+ 4 Player Co-Op 1CC Playthrough Divinde Difficulty Live Demonstration on Shmup Slam 3 | 37:19 |
| 48. | Gaming With BenWo0 | 39 | Ben Plays (Blind) - Jamestown | 18:17 |
| 49. | UnauthorizedAccessGaming | 34 | Unauthorized Access: Let's Play Jamestown | 34:31 |
| 50. |  Aku | 33 | JAMESTOWN+ (Nintendo Switch) Full Playthrough | 37:28 |
| 51. | VideoGameObsession | 32 | Let's Play (no talk) - Jamestown+ - An awesome vertical shooter on the PlayStation 4 | 15:01 |
| 52. | Marstead | 31 | ✔️ Full Blind Playthrough (Jamestown: Legend of the Lost Colony) | 1:55:53 |
| 53. |  The Foreseen Arcade | 30 | Arcade Archives: Moon Cresta - PlayStation 4 Gameplay | 6:36 |
| 54. | Super Retro Theater | 27 | Let's Play - Jamestown+ (Stream Record Test) | 1:15:47 |
| 55. | Let's Play The World! | 25 | Let's Play! - Steam Roll - 15 - Jamestown | 16:09 |
| 56. | Video Game World | 21 | Jamestown+ Gameplay 3 PlayStation 4 (PS4) | 5:43 |
| 57. |  ocg obscure | 13 | Jamestown playthrough: Defiance in the Black Garrison | 10:53 |
| 58. | aboyandhisdogs | 13 | Let's Play Jamestown (PC) - Mission 5 | 9:25 |
| 59. | 3MAALP | 12 | Jamestown: Legend Of The Lost Colony ~ Yellow Lazer Beam ~ 3MAALP | 10:58 |
| 60. | Andy Plays All Night | 11 | SO THIS IS BRITISH COLONIAL MARS | Let's Play: Jamestown+ | 34:45 |
| 61. | Longplay Studio 313 | 9 | Jamestown+ Longplay (Playstation 4) | 46:36 |
| 62. | Expansive | 8 | Jamestown Plus - Playstation 4 Teaser | 1:29 |
| 63. | ASPG Gaming | 7 | Polo plays Jamestown + 21.04.2015 | 1:49:17 |
| 64. | OnPSX | 7 | Jamestown Plus - Release Trailer (PS4) | 1:05 |
| 65. | Zero2k0 | 4 | Jamestown+- (Playstation 4) | 1:21:22 |
| 66. | Soapie Plays | 3 | DODGEEEEEEE | Soapie Plays New Games: Jamestown | 1:11:56 |
| 67. | Chronno S. Trigger | 2 | Chronno Plays Jamestown | 17:07 |
| 68. |  PC do Paulo | 1 | Jamestown+ - PS4 - Gameplay | 12:50 |
| 69. | Nado Cato | 0 | PlayStation 4 Pro Game Play | 9:54 |
