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0TaqMan PCR for Accurate Diagnosis of Tick-Transmitted Pathogens in Cattle | Research Study from Kerala, India
🔬 About This Study:
This video discusses a research study that utilized TaqMan-based qualitative Real-Time PCR (qPCR) for the accurate and confirmatory diagnosis of multiple tick-transmitted pathogens affecting the bovine population in Kerala, India. Conventional microscopy-based methods often fail to identify pathogens at the species level, especially when they have similar infective stages. This study highlights the advantages of molecular diagnostics in detecting co-infections and secondary pathogens that impact cattle health.
📌 Key Findings:
Sample Collection: 60 peripheral blood samples (EDTA) were randomly collected from five private cattle farms in Wayanad & Thrissur districts, Kerala.
Pathogens Detected: The study identified Theileria spp., Mycoplasma haemocanis, and Pasteurella multocida using highly sensitive TaqMan qPCR.
Ct (Cycle Threshold) Values:
Theileria genus: Avg Ct = 12.79 (Range: 4.1–36.19)
M. haemocanis: Avg Ct = 22.42 (Range: 2.66–37.6)
P. multocida: Avg Ct = 20.20 (Range: 2.74–35.72)
(Lower Ct = Higher pathogen load in initial sample)
P. multocida as a Secondary Pathogen: Found to cause severe issues due to immune suppression by primary tick-borne infections.
💡 Implications for Cattle Health Management:
Vaccination Strategies: Co-infections may interfere with seroconversion & duration of immunity, requiring tailored vaccination programs.
Need for Advanced Diagnostics: Microscopy alone is insufficient; molecular methods like TaqMan PCR improve detection accuracy.
📄 Methodology:
DNA extraction: High Pure PCR Template Preparation Kit
Primer efficiency tested via standard curve using custom-synthesized positive clones
Cycling conditions optimized for each pathogen
📍 Why This Matters?
Tick-borne diseases severely impact cattle productivity. This study emphasizes the need for advanced diagnostic tools like TaqMan qPCR to detect multiple pathogens simultaneously, aiding in better treatment and vaccination planning by the Department of Animal Husbandry, Kerala.
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TaqMan PCR
Real-Time PCR
Tick-borne pathogens
Bovine diseases
Cattle health
Theileria detection
Mycoplasma haemocanis
Pasteurella multocida
Molecular diagnostics in veterinary science
qPCR in cattle
Tick-transmitted diseases
Cattle co-infections
Veterinary PCR testing
Diagnosis of bovine pathogens
Ct values in qPCR
Kerala cattle farming
Wayand and Thrissur veterinary study
High Pure PCR kit
Primer efficiency testing
Secondary infections in cattle
How does TaqMan PCR work for tick diseases?
Why is microscopy not enough for pathogen detection?
Impact of P. multocida in tick-infected cattle
Best diagnostic methods for bovine tick diseases
Vaccination challenges in co-infected cattle