| 1. |  Daz Games | Meet Choo Choo Charles! Terrifying! | 1,702 | Let's Play |
| 2. | GamerGoku | Goku Plays Choo Choo Charles | SPIDER TRAIN! | 316 | Let's Play |
| 3. | SuperHorrorBro | The Baby Spider Trains Have Hatched || Choo Choo Charles: The Forgotten Ones (Playthrough) | 301 | Let's Play |
| 4. | Forsen Plays | Forsen Plays Choo-Choo Charles (With Chat) | 39 | Let's Play |
| 5. | Beardo Benjo | This SPIDER TRAIN horror game is NIGHTMARE FUEL! // Choo Choo Charles Full Game | 22 | Let's Play |
| 6. | PinkStylistPlays | DON'T TOUCH CHARLES'S EGGS! | Choo Choo Charles | Game Play Part 3 | 18 | Let's Play |
| 7. | Lambs Life | spider train what could go wrong | choo choo Charles | full play through | 9 | Let's Play |
| 8. | Channel 13: Horror | Choo Choo Charles Let's Play With ScaredyMat | 9 | Let's Play |
| 9. | Dak Dak | Choo-Choo Charles - Let's Play! - The train of destiny! - Ep 1 | 8 | Let's Play |
| 10. | SquishyCabbage | THIS ISLAND IS FULL OF HORRORS | Choo Choo Charles (Full Playthrough Part 2) | 4 | Let's Play |
| 11. | ScottyDGaming | Choo Choo Charles, Part 3 / The Temple, Last Boss, Ending | 4 | Let's Play |
| 12. | LamerGamer | Choo Choo Charles Playthrough Highlights | 4 | Let's Play |
| 13. | ScottyDGaming | Choo Choo Charles, Part 1 / Thomas the Tank Aint got Shiiiiiiii (Full game First Hour Intro) | 3 | Let's Play |
| 14. | ScottyDGaming | Choo Choo Charles, Part 2 / Haunted Pages, The Second Magical Egg, Mines, Lighthouse | 3 | Let's Play |
| 15. | Kero | Choo Choo Charles Wants Me Badly!! #shorts | 3 | Let's Play |
| 16. | Shadow Daddy M2 | Tonight: CHOO CHOO CHARLES Full Playthrough - SCREAMING EDITION! | 2 | Let's Play |
| 17. | Dak Dak | Choo-Choo Charles - Let's Play! - Lookin' good! - Ep 4 | 2 | Let's Play |
| 18. | Caztecx | Choo-Choo Charles | FULL PLAYTHROUGH! | Caztecx LIVE | 2 | Let's Play |
| 19. | Dak Dak | Choo-Choo Charles - Let's Play! - Helping out the locals - Ep 2 | 2 | Let's Play |
| 20. | Kero | Choo Choo Charles Is Finally Unleashed!! // Choo Choo Charles | 2 | Let's Play |
| 21. | ScaredForScience | Choo Choo Charles | Blind Playthrough | #1 All Aboard! | 2 | Let's Play |
| 22. | Dak Dak | Choo-Choo Charles - Let's Play! - He's so mad!!! - Ep 5 | 2 | Let's Play |
| 23. | Beeb1up Gaming | Choo-Choo Charles Playthrough Episode 4 (We Killed Charles! But It's Not Over...) | 2 | Let's Play |
| 24. | Soul_Render | Spider? trains?... wait what // FULL PLAYTHROUGH: choo choo charles | 1 | Let's Play |
| 25. | Valentine Play | No-Snake Hotel - Mini Horror Game - Full Playthrough | 1 | Let's Play |
| 26. | Kero | My 1st Death To Choo Choo Charles 💀 #shorts | 1 | Let's Play |
| 27. | SquishyCabbage | YOU WON'T BELIEVE WHAT HAPPENS AT THE END | Choo Choo Charles (Full Playthrough Part 3) | 1 | Let's Play |
| 28. | SquishyCabbage | NIGHTMARE TRAIN | Choo Choo Charles (Full Playthrough Part 1) | 1 | Let's Play |
