| 1. |  Daz Games | Meet Choo Choo Charles! Terrifying! | 1,310,589 | Let's Play |
| 2. | GamerGoku | Goku Plays Choo Choo Charles | SPIDER TRAIN! | 384,035 | Let's Play |
| 3. | SuperHorrorBro | The Baby Spider Trains Have Hatched || Choo Choo Charles: The Forgotten Ones (Playthrough) | 380,945 | Let's Play |
| 4. | Beardo Benjo | This SPIDER TRAIN horror game is NIGHTMARE FUEL! // Choo Choo Charles Full Game | 9,461 | Let's Play |
| 5. | Forsen Plays | Forsen Plays Choo-Choo Charles (With Chat) | 9,400 | Let's Play |
| 6. | Channator Gaming | Choo-Choo Charles - The Train Out of Your Nightmares 😱 | + Chan Reacts | | 2,168 | Let's Play |
| 7. | PinkStylistPlays | DON'T TOUCH CHARLES'S EGGS! | Choo Choo Charles | Game Play Part 3 | 2,069 | Let's Play |
| 8. | Channel 13: Horror | Choo Choo Charles Let's Play With ScaredyMat | 1,645 | Let's Play |
| 9. | Kero | My 1st Death To Choo Choo Charles 💀 #shorts | 1,528 | Let's Play |
| 10. | Kero | Choo Choo Charles Wants Me Badly!! #shorts | 700 | Let's Play |
| 11. | LamerGamer | Choo Choo Charles Playthrough Highlights | 626 | Let's Play |
| 12. | Halotrooper001 | Choo-Choo Charles (Blind Playthrough) Stream 2 | 346 | Let's Play |
| 13. | SquishyCabbage | NIGHTMARE TRAIN | Choo Choo Charles (Full Playthrough Part 1) | 295 | Let's Play |
| 14. | Caztecx | Choo-Choo Charles | FULL PLAYTHROUGH! | Caztecx LIVE | 260 | Let's Play |
| 15. | ScottyDGaming | Choo Choo Charles, Part 3 / The Temple, Last Boss, Ending | 238 | Let's Play |
| 16. | SquishyCabbage | YOU WON'T BELIEVE WHAT HAPPENS AT THE END | Choo Choo Charles (Full Playthrough Part 3) | 233 | Let's Play |
| 17. | ScottyDGaming | Choo Choo Charles, Part 2 / Haunted Pages, The Second Magical Egg, Mines, Lighthouse | 231 | Let's Play |
| 18. | ScottyDGaming | Choo Choo Charles, Part 1 / Thomas the Tank Aint got Shiiiiiiii (Full game First Hour Intro) | 171 | Let's Play |
| 19. | ScaredForScience | Choo Choo Charles | Blind Playthrough | #1 All Aboard! | 156 | Let's Play |
| 20. | Dak Dak | Choo-Choo Charles - Let's Play! - The train of destiny! - Ep 1 | 98 | Let's Play |
| 21. | Lambs Life | spider train what could go wrong | choo choo Charles | full play through | 97 | Let's Play |
| 22. | Dak Dak | Choo-Choo Charles - Let's Play! - He's so mad!!! - Ep 5 | 85 | Let's Play |
| 23. | Valentine Play | No-Snake Hotel - Mini Horror Game - Full Playthrough | 75 | Let's Play |
| 24. | Kero | Choo Choo Charles Is Finally Unleashed!! // Choo Choo Charles | 56 | Let's Play |
| 25. | SquishyCabbage | THIS ISLAND IS FULL OF HORRORS | Choo Choo Charles (Full Playthrough Part 2) | 55 | Let's Play |
| 26. | ItsMartyy_ | Choo-Choo Charles I Full Playthrough I New Indie Horror Game | 46 | Let's Play |
| 27. | Beeb1up Gaming | Choo-Choo Charles Playthrough Episode 1 (Charles Be Angry From The Start!) | 44 | Let's Play |
| 28. | Dak Dak | Choo-Choo Charles - Let's Play! - Lookin' good! - Ep 4 | 40 | Let's Play |
| 29. | Halotrooper001 | Choo-Choo Charles (Blind Playthrough) Stream 1 | 37 | Let's Play |
| 30. | Beeb1up Gaming | Choo-Choo Charles Playthrough Episode 3 (We Set Up A Trap On The Bridge!) | 32 | Let's Play |
| 31. | Dak Dak | Choo-Choo Charles - Let's Play! - Helping out the locals - Ep 2 | 31 | Let's Play |
| 32. | Dak Dak | Choo-Choo Charles - Let's Play! - Heeeeeere comes Charles! - Ep 3 | 28 | Let's Play |
| 33. | Shadow Daddy M2 | Tonight: CHOO CHOO CHARLES Full Playthrough - SCREAMING EDITION! | 24 | Let's Play |
| 34. | Beeb1up Gaming | Choo-Choo Charles Playthrough Episode 2 (There's A Spooky Ghost Out To Get Me!) | 22 | Let's Play |
| 35. | Soul_Render | Spider? trains?... wait what // FULL PLAYTHROUGH: choo choo charles | 21 | Let's Play |
| 36. | Beeb1up Gaming | Choo-Choo Charles Playthrough Episode 4 (We Killed Charles! But It's Not Over...) | 17 | Let's Play |
| 37. | Man In Shorts - Minecraft | OctolingNinjala2017 Reacts To Mario Plays: CHOO CHOO CHARLES By @theawesomarioYT | 10 | Let's Play |
