| 1. |  Markiplier | I'M GONNA KILL A BUILDING | 64,848 | |
| 2. |  Baitybait | El MEJOR juego de cartas - Slay the Spire | 52,543 | |
| 3. | IGN | Slay the Spire Developers React to 5 Minute Speedrun | 47,255 | |
| 4. |  RTGame | An Increasingly Stupid Session of Slay the Spire | 38,577 | |
| 5. | jacksepticeye | YOU TRIGGERED MY TRAP CARD | Slay The Spire #1 | 35,064 | |
| 6. |  The Spiffing Brit | The Worlds Hardest Video Game Challenge - Is Actually Super Easy... | 28,167 | |
| 7. | jacksepticeye | BOSS SLAYER | Slay The Spire #2 | 25,777 | |
| 8. | Game Maker's Toolkit | How Synergies Make Slay the Spire Fun | 23,114 | |
| 9. | MagikarpUsedFly | I tried | 21,938 | |
| 10. | Northernlion | Slay the Spire - Northernlion Plays - Episode 1 | 17,613 | Let's Play |
| 11. |  tvgry | Jak poświęciłem 50 godzin, by zabić jednego bossa | 16,753 | |
| 12. | Frost Prime | The GREATEST Slay The Spire run EVER | 11,662 | |
| 13. |  HandOfUncut | Der beste Run meines Lebens | Slay the Spire | 11,651 | |
| 14. | Not Albino | Slay the Spire - IronChad | 11,594 | |
| 15. | MagikarpUsedFly | I'm still trying | 11,518 | |
| 16. | ТАК ОСТРО! | Новый Fallout, скандал Star Wars Outlaws, конец Dead Space, анонс Slay the Spire 2. Игровые новости! | 11,579 | |
| 17. |  Chloe ch. 沙花叉クロヱ - holoX - | 【Slay the Spire】時間がめちゃめちゃ溶ける伝説の神ゲーで遊ぶ！【沙花叉クロヱ/ホロライブ】 | 10,481 | |
| 18. | Pekora Ch. 兎田ぺこら | 【slay the spire】デュエルスタンバイ！！！！！！！！！！！！！！！！！！！ぺこ！【ホロライブ/兎田ぺこら】 | 10,917 | |
| 19. | Hutts | Isaac in Slay the Spire! (Completely Broken) | 10,101 | |
| 20. | RedRiotRoss | How 2 Slay the Spire | 9,811 | |
| 21. | Korone Ch. 戌神ころね | 【Slay the Spire】カードゲームのはじまりだぜ！！！！【戌神ころね/ホロライブ】 | 9,580 | |
| 22. | RiskyCB | 2 Card Slay the Spire Run - Consistent Turn 1 Infinite Combo | 8,823 | |
| 23. | Ars Technica | How Slay the Spire’s Original Interface Almost Killed the Game | War Stories | Ars Technica | 8,701 | |
| 24. |  Spuzie | ♥ FRESH PLEM - Sp4zie Weekly #64 | 8,644 | |
| 25. | Bob Lennon | PUISSANCE MAXIMALE !!! -Slay the Spire- Petit gameplay détente (avec Jehal & Bob Lennon) | 8,615 | |
| 26. | Not Albino | Defect is Fair and Balanced | 8,596 | |
| 27. | Not Albino | Minimalist Achievement is Pain | 8,555 | |
| 28. | Not Albino | Ascension 25 is Terrible | 8,538 | |
| 29. | Not Albino | Slap the Spire | 8,427 | |
| 30. | alexelcapo | EL MAESTRO DE LAS CARTAS - Slay the Spire - EP 1 | 8,417 | |
| 31. | Not Albino | Peak Defect Gameplay | 8,234 | |
| 32. | Not Albino | My Brain is Defect | 8,188 | |
| 33. | Adam Millard - The Architect of Games | What Makes a Great Deckbuilder? | 8,041 | |
| 34. | Northernlion | Northernlion Plays Slay the Spire's New Character - Episode 1: The Watcher | 7,847 | Let's Play |
| 35. | Takanashi Kiara Ch. hololive-EN | 【SLAY THE SPIRE】I will send him to the usual room! #kfp #キアライブ | 7,722 | |
| 36. | Frost Prime | What if you started with 8 Foreign Influences?! | 7,693 | |
| 37. | Not Albino | You Just Activated My Meteor Strike | 7,629 | |
| 38. |  iXBT games | Переходим на диету! Обзор Slay the Spire (iOS) | 7,620 | |
| 39. | Northernlion | Slay the Spire - Northernlion Plays - Episode 2 | 7,519 | Let's Play |
| 40. | PietSmiet | Nur 1 Versuch in Slay the Spire | 7,514 | |
| 41. | Takanashi Kiara Ch. hololive-EN | 【SLAY THE SPIRE】Need a WIN with the Ironclad!!! #kfp #キアライブ | 7,011 | |
| 42. | Korone Ch. 戌神ころね | 【Slay the Spire】一回力尽きたら即終了！いけるとこまで頑張ってみる！【戌神ころね/ホロライブ】 | 6,917 | |
| 43. | Not Albino | Least Calculated Spire Turn | 6,891 | |
| 44. |  DangerouslyFunny | You Guys Made Me Do This | 6,849 | |
| 45. | darkjay | Slay the Spire Speedrun: Ironclad any% 4:30 | 6,891 | |
| 46. | Northernlion | Slay the Spire - Northernlion Plays - Episode 3 | 6,810 | Let's Play |
| 47. | Takanashi Kiara Ch. hololive-EN | 【SLAY THE SPIRE】Slaying as the WATCHER for the first time! #kfp #キアライブ | 6,758 | |
| 48. | Frost Prime | I found one of the most BROKEN combos!! | 6,715 | |
| 49. | Northernlion | Slay the Spire - Northernlion Plays - Episode 576 [One] | 6,573 | Let's Play |
| 50. | ViteC ► Play | ШПИЛЬ БОГА РАНДОМА ► Slay The Spire |1| Прохождение | 6,563 | |
| 51. | Frost Prime | What if you started with 8 Storms?! | 6,454 | |
| 52. | Mr. Fruit | MY NEW FAVORITE GAME! | Slay the Spire | 6,411 | |
| 53. | Takanashi Kiara Ch. hololive-EN | 【SLAY THE SPIRE】Slaying it. For real this time! #kfp #キアライブ | 6,365 | |
| 54. | Frost Prime | What if you started with 8 Claws AGAIN?! | 6,342 | |
| 55. | ViteC ► Play | ШПИЛЬ ЗА НОВОГО ГЕРОЯ. СОЗЕРЦАЮЩАЯ ► Slay The Spire |12| Прохождение | 6,198 | |
| 56. | Frost Prime | This video will teach you how to play the Defect | 6,145 | Guide |
| 57. | Upper Echelon | Blowing Up a 150k SCAM | 6,138 | |
| 58. |  iRaphahell | Aruncam o privire peste SLAY THE SPIRE ! | 5,932 | |
| 59. | Frost Prime | I taught Jocat how to play Slay The Spire | 5,917 | Guide |
| 60. | Not Albino | *oneshots boss turn 1* | 6,100 | |
| 61. | Verac | Slay the Spire Review | "VERAC SELLS OUT" Edition™ ($$$) | 6,460 | Review |
| 62. | Northernlion | Northernlion Attempts the Indie Pentathlon (01/26/2019) | 5,701 | |
| 63. | Frost Prime | What if you played as Hexaghost?! | 5,674 | |
| 64. | Тиджой | Гайд: Элитные монстры // Slay the Spire | 5,607 | |
| 65. | Frost Prime | What if you started with 8 PRESSURE POINTS?! | 5,576 | |
| 66. | Frost Prime | THIS is a poison run | 5,528 | |
| 67. | Not Albino | POV: Free win | 6,387 | |
| 68. |  MaxPalaro | PERDI 240 HORAS NESSE GAME | Slay The Spire | 5,435 | |
| 69. | Solem Hearthstone | The Perfect Alternatives to Hearthstone | 5,321 | |
| 70. | Frost Prime | I ranked every card for the Ironclad in Slay The Spire | 5,312 | |
| 71. | ViteC ► Play | ГОЛОВО-ЛОМКА ► Slay The Spire |3| Прохождение | 5,254 | |
| 72. | Northernlion | SNKRX meets Slay the Spire? (Just King) | 5,243 | |
| 73. | Frost Prime | What if EVERY Floor Was An Elite?! | 5,149 | |
| 74. | Frost Prime | The most DISGUSTING Ironclad deck you've ever seen | 5,130 | |
| 75. | Тиджой | Рогалик для самых умных людей // Обзор Slay the Spire | 5,116 | |
| 76. | Northernlion | Northernlion Rates Every Ironclad Card in Slay the Spire! | 5,059 | |
| 77. | not Otzdarva | We BROKE this card in the funniest way... | Slay the Spire | 5,389 | |
| 78. | Not Albino | I let twitch chat pick my cards | 4,894 | |
| 79. | Frost Prime | You'll Never Believe How I Won The Hardest STS Challenge!! | 4,868 | |
| 80. | Frost Prime | What if EVERY Floor is a Question Mark?! | 4,865 | |
| 81. | IGN | Slay the Spire Review | 4,834 | Review |
| 82. | Not Albino | POV Pandoras Box | 4,753 | |
| 83. | Frost Prime | What if EVERY relic was Calling Bell?! | 4,737 | |
| 84. | Frost Prime | Teaching Jocat to play The Defect on A20 | 4,698 | |
| 85. | Frost Prime | Have you seen a cleaner Ironclad infinite?! | 4,692 | |
| 86. | Northernlion | Slay the Spire - Northernlion Plays - Episode 568 [Good Stuff] | 4,691 | Let's Play |
| 87. | ingeniousclown Gaming | Slay the Spire "Clones" are getting RIDICULOUS! | 4,686 | |
| 88. | Frost Prime | I ranked every Relic in Slay The Spire | 4,675 | |
| 89. | Not Albino | Weakest Defect Run | 7,114 | |
| 90. | Elira Pendora 【NIJISANJI EN】 | 【Slay the Spire】 heart of the cards 【NIJISANJI EN | Elira Pendora】 | 4,547 | |
| 91. | Revenant | SLAY THE SPIRE | La primera sorpresita del año? :D | 4,459 | |
| 92. | Frost Prime | Slay The Spire but I don't fight anyone (literally) | 4,443 | |
| 93. | Frost Prime | What if every relic was Chemical X but you start with a bottled Whirlwind?! | 4,437 | |
| 94. | Northernlion | My First Slay the Spire Video in YEARS | 4,415 | |
| 95. | Frost Prime | THIS IS THE BEST MODDED CHARACTER!! - Hermit | 4,407 | |
| 96. | Northernlion | Let's Look At: Slay the Spire! | 4,366 | |
| 97. | Frost Prime | What if you started with 9 Blade Dances?! | 4,355 | |
| 98. | Frost Prime | Does the watcher have lethal?! | 4,335 | |
| 99. | Frost Prime | What if you played as The Guardian?! | 4,311 | |
| 100. | Frost Prime | What if you started with 8 heel hooks?! | 4,299 | |
