| 1. |  xQc | xQc Plays The Binding of Isaac: Afterbirth+ | xQcOW | 220,820 | Let's Play |
| 2. | Jonus | Top 10 Binding of Isaac Afterbirth+ mods | 88,580 | Let's Play |
| 3. | Team Alpha | Alphabirth Enemy Showcase [Pack 1 & 2] | 78,905 | Let's Play |
| 4. | Team Alpha | Alphabirth: Mom's Closet [Release Trailer] | 19,732 | Let's Play |
| 5. | Kate Lovelymomo | The Binding of Isaac Antibirth Race with SurixPlays [Episode 1] | 11,728 | Let's Play |
| 6. | Team Alpha | Alphabirth: All That Is Unholy [Release Trailer] | 11,363 | Let's Play |
| 7. | Team Alpha | Alphabirth Item Guide: Possessed Shot | 9,118 | Let's Play |
| 8. | Kate Lovelymomo | The Binding of Isaac Afterbirth+ with Surixplays [Episode 4] (Re-up) | 7,524 | Let's Play |
| 9. | Team Alpha | Alphabirth Item Guide: Isaac's Skull | 7,454 | Let's Play |
| 10. | Team Alpha | Alphabirth Item Guide: Satan's Contract | 7,076 | Let's Play |
| 11. | Taste Gaming | THE BINDING OF ISAAC: REBIRTH - LUDOVICO - 9 LIVES - DARK BUM PLAYTHROUGH | 6,911 | Let's Play |
| 12. | Team Alpha | Alphabirth Item Guide: Blacklight | 6,731 | Let's Play |
| 13. | Team Alpha | Alphabirth Item Guide: Bugged Bombs | 6,285 | Let's Play |
| 14. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 07 - Everything's Terrible! (including Eve) | 6,109 | Let's Play |
| 15. | Team Alpha | Alphabirth Item Guide: The Cosmos | 6,007 | Let's Play |
| 16. | Team Alpha | Alphabirth Item Guide: Bionic Arm | 5,966 | Let's Play |
| 17. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 08 - Lucky Attractor | 5,827 | Let's Play |
| 18. | Team Alpha | Alphabirth Item Guide: Subconscious | 5,818 | Let's Play |
| 19. | Team Alpha | Alphabirth Item Guide: Tech Alpha | 5,763 | Let's Play |
| 20. | Team Alpha | Alphabirth Item Guide: Mr. Squishy | 5,748 | Let's Play |
| 21. | Team Alpha | Alphabirth Item Guide: Old Controller | 5,720 | Let's Play |
| 22. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 09 - A Boy and His Book | 5,624 | Let's Play |
| 23. | Kate Lovelymomo | The Binding of Isaac Antibirth Race with SurixPlays [Episode 2] | 5,603 | Let's Play |
| 24. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 10 - Blood Vomit Perfume | 5,555 | Let's Play |
| 25. | Team Alpha | Alphabirth Item Guide: Smart Bombs | 5,533 | Let's Play |
| 26. | Team Alpha | Alphabirth Item Guide: Stoned Buddy | 5,477 | Let's Play |
| 27. | Team Alpha | Alphabirth Item Guide: Furnace | 5,466 | Let's Play |
| 28. | Team Alpha | Alphabirth Item Guide: Crystallized | 5,458 | Let's Play |
| 29. | Team Alpha | Alphabirth Item Guide: Shooting Star | 5,449 | Let's Play |
| 30. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 12 - I Still Hate You | 5,397 | Let's Play |
| 31. | Team Alpha | Alphabirth Item Guide: Poly-Mitosis | 5,385 | Let's Play |
| 32. | Team Alpha | Alphabirth Item Guide: Abyss | 5,361 | Let's Play |
| 33. | Team Alpha | Alphabirth Item Guide: Peanut Butter | 5,289 | Let's Play |
| 34. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 11 - Perfect Fly | 5,260 | Let's Play |
| 35. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Episode 13 - ??? | 5,241 | Let's Play |
| 36. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 05 - The Tank | 5,185 | Let's Play |
| 37. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 06 - Solar System | 5,141 | Let's Play |
| 38. | Team Alpha | Alphabirth Item Guide: Graphics Error | 4,998 | Let's Play |
| 39. | Team Alpha | Alphabirth Item Guide: Green Candle | 4,939 | Let's Play |
| 40. | Kate Lovelymomo | The Binding of Isaac Afterbirth+ Race with SurixPlays [Episode 1] | 4,839 | Let's Play |
| 41. | Team Alpha | Alphabirth Item Guide: Humility | 4,824 | Let's Play |
| 42. | Team Alpha | Alphabirth Item Guide: Hive Head | 4,705 | Let's Play |
| 43. | Team Alpha | Alphabirth Item Guide: Lil Miner | 4,687 | Let's Play |
| 44. | Team Alpha | Alphabirth Item Guide: Aimbot | 4,646 | Let's Play |
| 45. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 10 - Cursed! | 4,560 | Let's Play |
| 46. | Team Alpha | Alphabirth Item Guide: Faithful Ambivalence | 4,546 | Let's Play |
| 47. | Team Alpha | Alphabirth Item Guide: Rocket Shoes | 4,531 | Let's Play |
| 48. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 04 - Darkness Falls | 4,487 | Let's Play |
| 49. | Team Alpha | Alphabirth Item Guide: Brown Eye (Trinket) | 4,484 | Let's Play |
| 50. | Team Alpha | Alphabirth Item Guide: Birth Control | 4,481 | Let's Play |
| 51. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 08 - Cat Got Your Tongue | 4,430 | Let's Play |
| 52. | Team Alpha | Alphabirth Item Guide: Spirit Eye | 4,404 | Let's Play |
| 53. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 09 - Demo Man | 4,381 | Let's Play |
| 54. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth #16 - Evil Technology | 4,291 | Let's Play |
| 55. | Team Alpha | Alphabirth Item Guide: Lil Alastor | 4,283 | Let's Play |
| 56. | Ramza411 | The Binding of Isaac: Rebirth - Run 4 - Stomping the Brown Snake | 4,270 | Let's Play |
| 57. | Team Alpha | Alphabirth Item Guide: Hot Coals | 4,219 | Let's Play |
| 58. | Graeme Games | Let's Play Riddle School 4 (Replay) | Flash Game Series | Graeme Games | 4,185 | Let's Play |
| 59. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth #14 - Welcome Back Eve | 4,154 | Let's Play |
| 60. | Team Alpha | Alphabirth Item Guide: Judas' Fez | 4,145 | Let's Play |
| 61. | Team Alpha | Alphabirth Item Guide: Owl Totem | 4,144 | Let's Play |
| 62. | Team Alpha | Alphabirth Item Guide: Paint Palette | 4,001 | Let's Play |
| 63. | Newfie Bangaa | Let's Play The Binding of Isaac: Rebirth #1 - Born Again | 3,937 | Let's Play |
| 64. | Team Alpha | Alphabirth Item Guide: Miniature Meteor | 3,862 | Let's Play |
| 65. | Team Alpha | Alphabirth Item Guide: Hemophilia | 3,839 | Let's Play |
| 66. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth #15 - My Only Friend | 3,781 | Let's Play |
| 67. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 12 - When Life Gives You Lemons | 3,780 | Let's Play |
| 68. | Team Alpha | Alphabirth Item Guide: Quill Feather | 3,746 | Let's Play |
| 69. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 07 - Suicide King | 3,717 | Let's Play |
| 70. | Team Alpha | Alphabirth Item Guide: Alastor's Candle | 3,682 | Let's Play |
| 71. | Team Alpha | Alphabirth Item Guide: Blooderfly | 3,651 | Let's Play |
| 72. | Kate Lovelymomo | The Binding of Isaac Antibirth Race with SurixPlays [Episode 4] | 3,608 | Let's Play |
| 73. | Team Alpha | Alphabirth Item Guide: Hushy Fly | 3,596 | Let's Play |
| 74. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 14 - It's in the Cards | 3,572 | Let's Play |
| 75. | michaelalfox | Foxman Plays: The Binding of Isaac: Rebirth - Ep. 1 - Slate | 3,570 | Let's Play |
| 76. | Team Alpha | Alphabirth Item Guide: Hoarder | 3,534 | Let's Play |
| 77. | Team Alpha | Alphabirth Item Guide: Book of the Dead | 3,507 | Let's Play |
| 78. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth - Challenge 13 - Beans! | 3,459 | Let's Play |
| 79. | Team Alpha | Alphabirth Item Guide: Stone Nugget | 3,289 | Let's Play |
| 80. | Team Alpha | Alphabirth Item Guide: Brunch | 3,281 | Let's Play |
| 81. | Team Alpha | Alphabirth Item Guide: Cracked Rock | 3,234 | Let's Play |
| 82. | Team Alpha | Alphabirth Item Guide: Infested Baby | 3,172 | Let's Play |
| 83. | Kate Lovelymomo | The Binding of Isaac Afterbirth+ Race with Surixplays [Episode 3] | 3,116 | Let's Play |
| 84. | Team Alpha | Alphabirth Item Guide: Infection | 3,007 | Let's Play |
| 85. | Kate Lovelymomo | The Binding of Isaac Afterbirth+ Race with Surixplays [Episode 2] | 2,985 | Let's Play |
| 86. | Team Alpha | Alphabirth Item Guide: Moon Rock (Trinket) | 2,961 | Let's Play |
| 87. | Danzel Glovington | Real Platinum God!! Steven! Getting 111% in the Binding of Isaac: Rebirth | 2,882 | Let's Play |
| 88. | Team Alpha | Alphabirth Item Guide: Endor's Hat | 2,870 | Let's Play |
| 89. | Ramza411 | The Binding of Isaac: Rebirth - Run 1 - A Great Start | 2,767 | Let's Play |
| 90. | Newfie Bangaa | Let's Play The Binding of Isaac: Afterbirth #1 - Coming Home | 2,716 | Let's Play |
| 91. | Joshua Kanai | GULPING ALL the TRINKETS | 2,578 | Let's Play |
| 92. | Psychedelic Eyeball | Let's Play Binding of Isaac Rebirth #17 - Antibirth | 2,401 | Let's Play |
| 93. | Team Alpha | Alphabirth Item Guide: Leaking Bombs | 2,275 | Let's Play |
| 94. | michaelalfox | Foxman Plays: The Binding of Isaac: Rebirth - Ep. 18 - Keep On and Buck Up | 2,118 | Let's Play |
| 95. | kintips | Review The Binding of Isaac Rebirth Sony Playstation Vita PSV PSN Free Nicalis Plus | 2,075 | Let's Play |
| 96. | Newfie Bangaa | Let's Play The Binding of Isaac: Rebirth #2 - Hubris | 2,027 | Let's Play |
| 97. | Phedran | The Binding of Isaac: Rebirth - 01 - Spoilers | 1,988 | Let's Play |
| 98. | Gorange | This Character Cries Whirlpools | 1,930 | Let's Play |
| 99. | Joshua Kanai | THIS is Why I LOVE Gulping TRINKETS: | 1,867 | Let's Play |
| 100. | Ramza411 | The Binding of Isaac: Rebirth - Run 2 - Eden, The Minigunner | 1,782 | Let's Play |
