| 1. |  Scott Manley | 233,623 | TIS-100 - The Assembly Language Puzzle Game That Nobody Asked For | 16:13 |
| 2. | Alex Diener | 13,179 | TIS-100 - E31: G.D$I.@SI (bonus) | 1:07:25 |
| 3. | Cyan Ch. 朝日小盐 | 3,919 | 【TIS-100】Get good at assembly! | VTuber Cyan | 0:00 |
| 4. | Game Wisdom | 1,949 | TIS-100 Spotlight and Tutorial | 1:08:01 |
| 5. | ZetaPlays | 1,274 | Let's Briefly Play TIS-100 - The Exposure Mask Viewer | 35:17 |
| 6. | Mafreidyne | 1,086 | [TIS-100] VTuber Struggles to Program in Assembly! | 02 | 57:17 |
| 7. | Evil Ryan | 941 | TIS-100 (Steam) - 39 Wave Collapse Supervisor (Nexus 24.511.7) | 2:48 |
| 8. | BasketMC | 884 | Sequence Indexer - TIS-100 - Programming Puzzles - Ep18 | 1:25:22 |
| 9. | Timothy Swan | 683 | TIS-100 Ep 1 Self-Test Diagnostic | 27:33 |
| 10. | Kinglink Reviews | 648 | TIS-100 - Quick Look Review - A review of pretty much every Zachtronics game as well | 9:54 |
| 11. | MemoryLeakDeath | 480 | Side Quests: TIS-100 | 1:00:04 |
| 12. | Brooks Builds (brookzerker) | 288 | Brooks plays TIS-100 | 4:48:09 |
| 13. | Achievement Hunter Xeinok | 247 | TIS-100 🏆 100% Achievement Playthrough | 42:37 |
| 14. | Daniel Dugovic | 187 | TIS-100 throws me for a LOOP | 1:32:04 |
| 15. | Guard13007 | 144 | Checking out... TIS-100 | 12:10 |
| 16. | Plus Two Comedy Gaming | 63 | A gift from Rasmus - Enigmatics Taskmaster | 49:56 |
| 17. | Allkeyshop | 54 | Top 15 Best Programming Games - December 2020 Selection | 5:07 |
| 18. | Porvelm | 24 | ive never hated a game more in my life | 2:10 |
| 19. | Roulette Productions | 15 | The Jaime Moreno Hour with TIS-100 | 55:00 |
| 20. | William Ferrell | 15 | TIS-100 - June 19, 2019 Livestream | 59:39 |
| 21. | Wool and Thyme | 13 | Continuing My Quest To Look Smart in TIS-100 and Also Some BeEES???? | 1:26:29 |
| 22. | Braden Best | 11 | When you go for the BUSY_LOOP achievement | 0:33 |
| 23. | JonathanIsBadAtGames | 11 | Entering the Matrix | TIS-100 | July 2016 | Humble Opinion | 5:39 |
| 24. | Androidx - Best Apps & Games | 7 | TIS-100 | Top 14 Alternatives of TIS-100 | 0:53 |
| 25. | Paradox GameStuff | 3 | TIS-100 // 5 | 1:00:53 |
