HoloCure - How to unlock the Pacifist achievement!
In order to unlock the Pacifist achievement, you have to let a boss stay alive for 5 minutes.
In order to easily do this, I would say get a few runs in and upgrade a few stats first. Things like speed and attack power will be very helpful. I had only done 4 runs at this point, so I wasn't far into the game yet (only beat the first 2 levels), so no need to max out your stats first unless you want to.
I would suggest choosing the first level, the Grassy Plains, when attempting this. It will be easiest since the monsters are pretty easy, and you have a huge playable area w/o many obstacles in the way.
Just keep playing and leveling up, and once the 20 minute mark hits, the boss will spawn in. Just run away from it and try to keep it off of the screen so you don't damage it. Be careful, though! The enemies all become very big as the time goes by! Keep running away while dodging enemies until the timer turns to minute 25, and the achievement will unlock!
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