| 1. |  Gab Smolders | 546,537 | [ Alien: Isolation ] 2nd Playthrough on HARD MODE - FINAL | 1:53:09 |
| 2. |  39daph vods | 119,756 | 39daph Plays Observation - Part 2 | 2:06:23 |
| 3. | Meghan Yeah | 111,041 | Alien: Isolation - Part 6 - Walkthrough / Playthrough / Let's Play | 5:39:30 |
| 4. |  Lavinia | 49,686 | The End -Ep 41 Let's Play: Alien Isolation | 22:26 |
| 5. | Eneija | 32,570 | Let's Play Alien: Isolation EP55 // STAIRWAY TO HAPPINESS [FINALE] | 26:41 |
| 6. | Eversor Fatalis | 10,078 | Let's Play Alien Isolation: Part 49 | 42:36 |
| 7. | Liara Gaming | 9,697 | Finale | Get to the Airlock - (26) Let's Play Alien: Isolation [Blind] | 36:26 |
| 8. | Liera | 9,330 | ENDING - Playing Alien Isolation For the First Time l Hard Mode - Pt.14 | 59:36 |
| 9. |  8 Bites | 8,067 | ALIEN ISOLATION BONUS EPISODE - Last Survivor | 20:23 |
| 10. | GamerKat09 | 6,202 | (P4) Let's Play - Perception [BLIND] - Surrounded by Dolls | 1:24:20 |
| 11. | Angel Alyx | 3,686 | HAPPY LATE HALLOWEEN | Alien Isolation LIVE | 3:40:04 |
| 12. |  GirlFromAus | 3,596 | Alien Isolation Playthrough Part 11 - An Insane Ending | 1:18:39 |
| 13. | More2Me 4Life | 1,637 | Alien Isolation Nightmare Challenge With Sound Alerts (2/2) | 6 Years on YouTube | Lets Play | 6:16:11 |
| 14. | GamerGirls | 1,218 | Alien Isolation Episode 44 - Back to the Beginning | 34:43 |
| 15. | KatFTWynn | 1,125 | Mother & Daughter Play: Alien Isolation: Crew Expendables DLC - ALIEN LOVES US ~Full Playthrough~ | 45:59 |
| 16. | DoubleDamsels | 900 | Double Damsels Play | Alien: Isolation | Part 8 | Two Weeks From Retirement | 10:23 |
| 17. | Celina VODs | 820 | I Won't Let the Milk Daddies Catch Me! - Alien: Isolation (Full Playthrough Part 2) | 8:02:11 |
| 18. | lilly waters | 480 | Get 'er Done Lets Play: Alien Isolation Part 37 | 37:36 |
| 19. | AssassinGlasgow | 428 | A Couple Let's Plays: Alien Isolation | ALIENS, ANDROIDS, NOOOOO [Pt. 3] | 15:04 |
| 20. | Unbealievable | 368 | Livestream Archive \\ Let's Play Alien: Isolation w/ My Girlfriend Janome \\ STOP BREATHING #3 | 1:32:16 |
| 21. | Usagisquared Plays | 108 | Usagisquared Plays Alien: Isolation - Ep 32 | 29:41 |
| 22. | Nashtya Newb | 58 | Nash plays her trap card in ALIEN: ISOLATION | Part 33 | 40:45 |
| 23. |  My Cinnamon Phoe | 29 | Phoephoe plays Alien: Isolation Nightmare Mode - Part 21 | 21:02 |
