| 1. |  Gab Smolders | 546,537 | [ Alien: Isolation ] 2nd Playthrough on HARD MODE - FINAL | 1:53:09 |
| 2. |  Meghan Yeah | 111,041 | Alien: Isolation - Part 6 - Walkthrough / Playthrough / Let's Play | 5:39:30 |
| 3. | 39daph vods | 103,519 | 39daph Plays Alien: Isolation - Part 2 (Final) | 7:41:48 |
| 4. | Pandorya | 96,728 | NARCOSIS #03 - Die GEISTER die ich rief.. ● Let's Play Narcosis | 28:50 |
| 5. |  Lavinia | 49,686 | The End -Ep 41 Let's Play: Alien Isolation | 22:26 |
| 6. | Eneija | 32,570 | Let's Play Alien: Isolation EP55 // STAIRWAY TO HAPPINESS [FINALE] | 26:41 |
| 7. | TheRPGMinx | 28,082 | I REALLY NEED A SPIDER DILD0 | Narcosis - Deep Sea Horror - FULL PLAYTHROUGH | 2:33:52 |
| 8. | Liera | 11,654 | ENDING - Playing Alien Isolation For the First Time l Hard Mode - Pt.14 | 59:36 |
| 9. | Liara Gaming | 11,432 | Running from the androids in Alien Isolation #shorts | 0:53 |
| 10. | Eversor Fatalis | 10,078 | Let's Play Alien Isolation: Part 49 | 42:36 |
| 11. | Geek Remix | 8,204 | REMEMBER ME 2 Girls 1 Let's Play Part 12: Mommy! | 21:49 |
| 12. |  8 Bites | 8,067 | ALIEN ISOLATION BONUS EPISODE - Last Survivor | 20:23 |
| 13. | GamerKat09 | 6,131 | (P17 FINAL) Let's Play - Alien: Isolation [BLIND] - Verlaine?? | 1:10:00 |
| 14. | Angel Alyx | 3,686 | HAPPY LATE HALLOWEEN | Alien Isolation LIVE | 3:40:04 |
| 15. |  GirlFromAus | 3,596 | Alien Isolation Playthrough Part 11 - An Insane Ending | 1:18:39 |
| 16. | More2Me 4Life | 1,637 | Alien Isolation Nightmare Challenge With Sound Alerts (2/2) | 6 Years on YouTube | Lets Play | 6:16:11 |
| 17. | GamerGirls | 1,218 | Alien Isolation Episode 44 - Back to the Beginning | 34:43 |
| 18. | Celina VODs | 934 | Will I Survive This? - Alien: Isolation (Full Playthrough Part 6) | 6:12:01 |
| 19. | DoubleDamsels | 900 | Double Damsels Play | Alien: Isolation | Part 8 | Two Weeks From Retirement | 10:23 |
| 20. | The RPG Chick | 623 | Let's Play Tacoma (Blind), Part 1 of 12: Docked! | 28:31 |
| 21. | lilly waters | 480 | Get 'er Done Lets Play: Alien Isolation Part 37 | 37:36 |
| 22. | AssassinGlasgow | 428 | A Couple Let's Plays: Alien Isolation | ALIENS, ANDROIDS, NOOOOO [Pt. 3] | 15:04 |
| 23. | Unbealievable | 368 | Livestream Archive \\ Let's Play Alien: Isolation w/ My Girlfriend Janome \\ STOP BREATHING #3 | 1:32:16 |
| 24. | Usagisquared Plays | 108 | Usagisquared Plays Alien: Isolation - Ep 32 | 29:41 |
| 25. | Nashtya Newb | 58 | Nash plays her trap card in ALIEN: ISOLATION | Part 33 | 40:45 |
| 26. |  My Cinnamon Phoe | 29 | Phoephoe plays Alien: Isolation Nightmare Mode - Part 21 | 21:02 |
